Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 35,373 G→C intergenic (‑2/+4) caiE ← / ← caiD stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091335,3730GC100.0% 151.3 / NA 43intergenic (‑2/+4)caiE/caiDstimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/20);  total (23/20)

CACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCT  >  NC_000913/35239‑35506
                                                                                                                                      |                                                                                                                                     
cacGCAGTGAGGCGAGTGGGCCGATATAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGcttgt                                                                                                                                   >  2:834174/1‑139 (MQ=255)
     aGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACg                                                                                                                              >  2:734232/1‑139 (MQ=255)
           gCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  2:107381/139‑1 (MQ=255)
           gCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                        <  1:805422/139‑1 (MQ=255)
                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             <  2:760678/112‑1 (MQ=255)
                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             >  1:760678/1‑112 (MQ=255)
                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                                  >  1:345822/1‑139 (MQ=255)
                                 cGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                                  >  1:356533/1‑139 (MQ=255)
                                    cTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCa                                                                                               <  2:438338/139‑1 (MQ=255)
                                    cTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCa                                                                                               >  1:438338/1‑139 (MQ=255)
                                                 aTCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGcc                                                                                  >  1:837837/1‑139 (MQ=255)
                                                  tCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGccc                                                                                 <  1:920998/139‑1 (MQ=255)
                                                   cGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCaa                                                                                              <  2:884970/125‑1 (MQ=255)
                                                   cGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCaa                                                                                              >  1:884970/1‑125 (MQ=255)
                                                    gCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCtt                                                                               <  2:630786/139‑1 (MQ=255)
                                                           aaGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAATGCCAGCGGCCCTTCAATGGc                                                                        <  1:443381/139‑1 (MQ=255)
                                                             gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                                      >  1:38775/1‑139 (MQ=255)
                                                                  cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                 <  2:240385/139‑1 (MQ=255)
                                                                   aCTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAg                                                                                       <  1:131600/116‑1 (MQ=255)
                                                                   aCTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAg                                                                                       >  2:131600/1‑116 (MQ=255)
                                                                        gATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGc                                                           <  2:345822/139‑1 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             >  1:322037/1‑69 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             <  2:322037/69‑1 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                         >  1:260541/1‑137 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                         <  2:260541/137‑1 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       >  1:679162/1‑139 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       >  2:461774/1‑139 (MQ=255)
                                                                            gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTCAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                       >  2:634600/1‑139 (MQ=255)
                                                                             aCAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAAc                                                      >  2:950940/1‑139 (MQ=255)
                                                                               aaaCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             <  1:1058661/66‑1 (MQ=255)
                                                                               aaaCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGt                                                                                                                             >  2:1058661/1‑66 (MQ=255)
                                                                                  cGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATg                                                 >  2:850838/1‑139 (MQ=255)
                                                                                      gTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                         >  2:1044454/1‑127 (MQ=255)
                                                                                      gTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                         <  1:1044454/127‑1 (MQ=255)
                                                                                       tCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAg                                            <  1:734232/139‑1 (MQ=255)
                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                >  2:1063844/1‑139 (MQ=255)
                                                                                                   ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                >  2:552523/1‑139 (MQ=255)
                                                                                                        aTTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCt                           <  2:837837/139‑1 (MQ=255)
                                                                                                                 tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCCTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAAtata                  >  2:939029/1‑139 (MQ=255)
                                                                                                                     aGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGGGTTTCAACACGCCGCTGCGAATATAGCGa              <  2:38775/139‑1 (MQ=255)
                                                                                                                           aaTAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGct        <  1:850838/139‑1 (MQ=255)
                                                                                                                                 tCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTtcttct  >  1:518705/1‑139 (MQ=255)
                                                                                                                                  cACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                                  >  1:853725/1‑42 (MQ=255)
                                                                                                                                  cACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCg                                                                                                  <  2:853725/42‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCT  >  NC_000913/35239‑35506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: