Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,247,620 C→T A279A (GCC→GCT malK → maltose ABC transportor ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,247,6200CT100.0% 77.2 / NA 25A279A (GCC→GCTmalKmaltose ABC transportor ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

GGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCCAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTC  >  NC_000913/4247486‑4247753
                                                                                                                                      |                                                                                                                                     
ggTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAata                                                                                                                                   <  2:358412/139‑1 (MQ=255)
   tCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGt                                                                                                                                >  2:28679/1‑139 (MQ=255)
        aaaGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTg                                                                                                                           <  1:1001897/139‑1 (MQ=255)
              gAACTTCCTGCCGGTAAAAGGGACTGCCACTGCAATCGAGCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGGCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTAtt                                                                                                                     <  2:206671/139‑1 (MQ=255)
                  ttCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGcc                                                                                                                 <  2:496380/139‑1 (MQ=255)
                   tCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGccc                                                                                                                <  1:4300/139‑1 (MQ=255)
                                            cGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCgg                                                                                                              >  1:268397/1‑116 (MQ=255)
                                            cGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCgg                                                                                                              <  2:268397/116‑1 (MQ=255)
                                                               gTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAgg                                                                    >  1:524995/1‑139 (MQ=255)
                                                               gTGGAGCTGCCGATACCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAgg                                                                    >  2:400008/1‑139 (MQ=255)
                                                                   aGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGa                                                                >  1:923249/1‑139 (MQ=255)
                                                                         cGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTAc                                                                                                     <  2:938953/96‑1 (MQ=255)
                                                                         cGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTAc                                                                                                     >  1:938953/1‑96 (MQ=255)
                                                                                  aTCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGa                                                 >  2:365868/1‑139 (MQ=255)
                                                                                             gTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCt                                                                                                                            >  1:923383/1‑53 (MQ=255)
                                                                                             gTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCt                                                                                                                            <  2:923383/53‑1 (MQ=255)
                                                                                                     gCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGa                                                                      >  2:1791/1‑99 (MQ=255)
                                                                                                     gCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGa                                                                      <  1:1791/99‑1 (MQ=255)
                                                                                                       cAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCa                            <  1:400008/139‑1 (MQ=255)
                                                                                                             aaaGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCa                      <  1:365868/139‑1 (MQ=255)
                                                                                                                  cGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATcc                 <  2:923249/139‑1 (MQ=255)
                                                                                                                   gTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCa                >  2:422208/1‑139 (MQ=255)
                                                                                                                    tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                   <  1:779256/55‑1 (MQ=255)
                                                                                                                    tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                   >  2:779256/1‑55 (MQ=255)
                                                                                                                                 ggAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTc  >  1:239682/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCCAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTC  >  NC_000913/4247486‑4247753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: