| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,254,003 | C→T | intergenic (+115/+40) | ubiA → / ← plsB | p‑hydroxybenzoate octaprenyltransferase/glycerol‑3‑phosphate O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,254,003 | 0 | C | T | 97.6% | 124.3 / ‑4.9 | 41 | intergenic (+115/+40) | ubiA/plsB | p‑hydroxybenzoate octaprenyltransferase/glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (19/21); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGT > NC_000913/4253885‑4254139 | cTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTcgccgc < 2:668803/139‑1 (MQ=255) tAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCaa < 2:1008382/139‑1 (MQ=255) catcCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGTAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCt < 1:441531/139‑1 (MQ=255) tcttctGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCg > 2:463041/1‑139 (MQ=255) tcttctGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCg > 2:146539/1‑139 (MQ=255) tGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTc < 1:146539/139‑1 (MQ=255) ggggcTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACg < 2:996248/136‑1 (MQ=255) ggcgCTTCTCAGGCCTACACAACACGTCGCATTTTATTGAATTTGCAGATTATGGAAGGCTGGATAAgg < 1:717978/69‑1 (MQ=255) ggcgCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAgg > 2:717978/1‑69 (MQ=255) ggcgCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCCTCGCCCTGCGTCGCACTCTCAATCGTCAAACg < 1:536369/139‑1 (MQ=255) gcgcTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGc < 2:413448/139‑1 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCa < 1:319859/72‑1 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCa > 2:319859/1‑72 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATtct > 1:753595/1‑77 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATtct < 2:753595/77‑1 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACg > 2:49792/1‑122 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACg < 1:49792/122‑1 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTg > 1:688873/1‑130 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTg < 2:688873/130‑1 (MQ=255) cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCa > 2:265451/1‑139 (MQ=255) acacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTc < 1:323577/139‑1 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCg < 1:712709/64‑1 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCg > 2:712709/1‑64 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCt > 1:1009399/1‑92 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCt < 2:1009399/92‑1 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTc > 1:984971/1‑97 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTc < 2:984971/97‑1 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTcc > 1:793432/1‑139 (MQ=255) cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTcc > 1:431721/1‑139 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGt > 1:30939/1‑91 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGt < 2:30939/91‑1 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCActct < 1:917540/99‑1 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCActct > 2:917540/1‑99 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt < 2:971776/132‑1 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt > 1:971776/1‑132 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAg > 1:208121/1‑139 (MQ=255) cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAg > 1:492473/1‑139 (MQ=255) ttATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACg > 1:941051/1‑94 (MQ=255) ttATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACg < 2:941051/94‑1 (MQ=255) ggCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCgg < 2:793432/139‑1 (MQ=255) gCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCCTCGTTTCTGCCGGt > 1:770392/1‑139 (MQ=255) | CTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGT > NC_000913/4253885‑4254139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |