Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,280,033	G→A	G18G (GGG→GGA)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,280,033	0	G	A	100.0%	205.0 / NA	63	G18G (GGG→GGA)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/29);  total (34/29)

CTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCG  >  NC_000913/4279898‑4280168                                                                                                                                        |                                                                                                                                        cTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGagtg                                                                                                                                      <  1:839270/139‑1 (MQ=255)       cGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                                <  2:143146/139‑1 (MQ=255)                gAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATg                                                                                                                       <  1:882048/139‑1 (MQ=255)                                      tAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgcttccgc                                                                                                 >  2:977282/1‑139 (MQ=255)                                                   aGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGc                                                                                    >  2:377569/1‑139 (MQ=255)                                                     aTCATAAAAAACATCGCAAACAGGGAGCGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCa                                                                                  >  2:145355/1‑139 (MQ=255)                                                               aCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCa                                                                                                                         <  2:381752/90‑1 (MQ=255)                                                               aCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCa                                                                                                                         >  1:381752/1‑90 (MQ=255)                                                               aCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATg                                                                                                                       >  2:924960/1‑92 (MQ=255)                                                               aCATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATg                                                                                                                       <  1:924960/92‑1 (MQ=255)                                                                  tCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCa                                                                                                               <  2:356565/97‑1 (MQ=255)                                                                  tCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCa                                                                                                               >  1:356565/1‑97 (MQ=255)                                                                   cGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTg                                                                    >  1:886775/1‑139 (MQ=255)                                                                                cGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTa                                                                       >  1:760414/1‑123 (MQ=255)                                                                                cGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCGTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTa                                                                       <  2:760414/123‑1 (MQ=255)                                                                                  cATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgttt                                                     >  2:736328/1‑139 (MQ=255)                                                                                    tGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                                <  2:785313/62‑1 (MQ=255)                                                                                    tGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                                >  1:785313/1‑62 (MQ=255)                                                                                         aTCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGc                                              <  1:481778/139‑1 (MQ=255)                                                                                            ttcttcACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtg                                           <  1:145355/139‑1 (MQ=255)                                                                                             tcttcACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGTAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtgt                                          <  1:348457/139‑1 (MQ=255)                                                                                                    cATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtgtg                                         >  1:208589/1‑133 (MQ=255)                                                                                                    cATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtgtg                                         <  2:208589/133‑1 (MQ=255)                                                                                                    cATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGttt                                   >  2:540477/1‑139 (MQ=255)                                                                                                       aCTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGcc                                                                                                                     <  1:357462/54‑1 (MQ=255)                                                                                                       aCTGATAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGcc                                                                                                                     >  2:357462/1‑54 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCAt                                                                                                                        <  1:244443/47‑1 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCAt                                                                                                                        >  2:244443/1‑47 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgct                                                                                                      <  1:185363/65‑1 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCccgct                                                                                                      >  2:185363/1‑65 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                            >  1:768374/1‑91 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                            >  2:705480/1‑91 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                            <  1:705480/91‑1 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTg                            >  2:713642/1‑139 (MQ=255)                                                                                                           aTAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTg                            >  2:13952/1‑139 (MQ=255)                                                                                                           aTAATGACCCTCGTAGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                            <  2:768374/91‑1 (MQ=255)                                                                                                            tAATGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGa                           >  1:40841/1‑139 (MQ=255)                                                                                                              aTGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                                >  1:240844/1‑36 (MQ=255)                                                                                                              aTGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACt                                                                                                                                <  2:240844/36‑1 (MQ=255)                                                                                                               tGACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAAct                        <  1:977282/139‑1 (MQ=255)                                                                                                                gACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAActc                       >  2:701168/1‑139 (MQ=255)                                                                                                                gACCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAActc                       >  2:951974/1‑139 (MQ=255)                                                                                                                 aCCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGcc                                                                                                                     >  2:470120/1‑44 (MQ=255)                                                                                                                 aCCCTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGcc                                                                                                                     <  1:470120/44‑1 (MQ=255)                                                                                                                  cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTg                                                                            <  1:650888/84‑1 (MQ=255)                                                                                                                  cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCtt                     >  1:241084/1‑139 (MQ=255)                                                                                                                  cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCATGAACTCtt                     >  1:559292/1‑139 (MQ=255)                                                                                                                  cccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATAGGTg                                                                            >  2:650888/1‑84 (MQ=255)                                                                                                                   ccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgtttggtt                                                 <  1:1041224/110‑1 (MQ=255)                                                                                                                   ccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACgtttggtt                                                 >  2:1041224/1‑110 (MQ=255)                                                                                                                   ccTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCttt                    >  1:210806/1‑139 (MQ=255)                                                                                                                    cTCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCtttt                   <  2:210806/139‑1 (MQ=255)                                                                                                                     tCGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCttttt                  >  1:361950/1‑139 (MQ=255)                                                                                                                      cGTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTa                 <  1:701168/139‑1 (MQ=255)                                                                                                                       gTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtg                                           >  2:295750/1‑112 (MQ=255)                                                                                                                       gTGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCAtg                                           <  1:295750/112‑1 (MQ=255)                                                                                                                        tGGTCTCGCTGTCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGt               <  1:713642/139‑1 (MQ=255)                                                                                                                                 tGTCCGGAGTGGTCACTCGTGTCATGTCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTAt      >  1:675016/1‑139 (MQ=255)                                                                                                                                   tCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAAt                                                                                       <  2:475145/56‑1 (MQ=255)                                                                                                                                   tCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAAt                                                                                       >  1:475145/1‑56 (MQ=255)                                                                                                                                   tCCGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATcc    <  1:13952/139‑1 (MQ=255)                                                                                                                                     cGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTgg                                                              <  2:954042/79‑1 (MQ=255)                                                                                                                                     cGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTgg                                                              >  1:954042/1‑79 (MQ=255)                                                                                                                                     cGGAGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCccg  <  2:40841/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATCCCGCTTCCGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCG  >  NC_000913/4279898‑4280168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑