Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,291,201 C→T G242G (GGC→GGT)  nrfD → formate‑dependent nitrite reductase, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,291,2010CT85.3% 74.2 / 5.6 36G242G (GGC→GGT) nrfDformate‑dependent nitrite reductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (15/14);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAA  >  NC_000913/4291067‑4291301
                                                                                                                                      |                                                                                                    
cTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATg                                                                                                  <  1:866653/139‑1 (MQ=255)
               aTACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGAGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTg                                                                                   >  2:258243/1‑139 (MQ=255)
                 aCGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGG‑GATGACGGTAAAGTTCGTgcgc                                                                                >  1:799742/1‑139 (MQ=255)
                       aCGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATGACGGTAAAGTTCGTGCGCtggtg                                                                           >  1:186308/1‑139 (MQ=255)
                                  cGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGG‑GATGACGGTAAAGTTCGTGCGATGGTGGCGGCATTAGGt                                                               <  2:799742/139‑1 (MQ=255)
                                    cATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTg                                                              >  1:846209/1‑139 (MQ=255)
                                                     gTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACgtgg                                             <  2:846209/139‑1 (MQ=255)
                                                     gTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACgtgg                                             <  2:799298/139‑1 (MQ=255)
                                                          tACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGtt                                        <  1:73698/139‑1 (MQ=255)
                                                          tACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGtt                                        <  1:258243/139‑1 (MQ=255)
                                                                 tATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGtt                                        <  2:10765/132‑1 (MQ=255)
                                                                 tATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGtt                                        >  1:10765/1‑132 (MQ=255)
                                                                         tCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGtt                                        >  1:101363/1‑124 (MQ=255)
                                                                         tCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGtt                                        <  2:101363/124‑1 (MQ=255)
                                                                         tCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGc                         >  2:123057/1‑139 (MQ=255)
                                                                             tgtgGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGAt                                                                                                   >  2:589350/1‑61 (MQ=255)
                                                                             tgtgGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGAt                                                                                                   <  1:589350/61‑1 (MQ=255)
                                                                             tgtgGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCggg                     >  2:1070469/1‑139 (MQ=255)
                                                                                   aTGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               <  2:186308/139‑1 (MQ=255)
                                                                                         tGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCg                                                           <  2:490264/89‑1 (MQ=255)
                                                                                         tGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCg                                                           >  1:490264/1‑89 (MQ=255)
                                                                                           aaaTCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATtgt       >  2:190734/1‑139 (MQ=255)
                                                                                            aaTCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               >  2:508590/1‑130 (MQ=255)
                                                                                            aaTCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               <  1:508590/130‑1 (MQ=255)
                                                                                             aTCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGc     <  1:1070469/139‑1 (MQ=255)
                                                                                                ttCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTAATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCaa  >  2:239563/1‑139 (MQ=255)
                                                                                                 tCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGc                                                            >  2:123547/1‑80 (MQ=255)
                                                                                                 tCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGc                                                            <  1:123547/80‑1 (MQ=255)
                                                                                                     gctgGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGa                                                  >  1:988297/1‑86 (MQ=255)
                                                                                                     gctgGTGGCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGa                                                  <  2:988297/86‑1 (MQ=255)
                                                                                                          tggCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               >  2:42297/1‑116 (MQ=255)
                                                                                                          tggCGTTTTTTGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               <  1:42297/116‑1 (MQ=255)
                                                                                                                   ttGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggct                   >  1:915580/1‑103 (MQ=255)
                                                                                                                   ttGTCGGTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggct                   <  2:915580/103‑1 (MQ=255)
                                                                                                                         gTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               <  1:89947/101‑1 (MQ=255)
                                                                                                                         gTCTGGCGCTGGGTGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCgggctgggg               >  2:89947/1‑101 (MQ=255)
                                                                                                                                      |                                                                                                    
CTGATCGCCATGGCGATACGCCAACGCAGTAACCCGCATTCCACGGAAGCGCAGTTTGTACACCGTATGGAAATCCCTGTGGTATGGGGTGAAATCTTCCTGCTGGTGGCGTTTTTTGTCGGTCTGGCGCTGGGCGATGACGGTAAAGTTCGTGCGCTGGTGGCGGCATTAGGTGGCGGTTTCTGGACGTGGTGGTTCTGGCTTGGTGTCGCCGGGCTGGGGCTGATTGTGCCAA  >  NC_000913/4291067‑4291301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑