Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,240	A→G	I280T (ATC→ACC)	mdtO ←	membrane translocase (MDR) of MdtNOP efflux pump, PET family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,240	0	A	G	100.0%	192.4 / NA	54	I280T (ATC→ACC)	mdtO	membrane translocase (MDR) of MdtNOP efflux pump, PET family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

CATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCAT  >  NC_000913/4302104‑4302362                                                                                                                                         |                                                                                                                           cATCTGACCCAGTTGTGACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTACCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGtt                                                                                                                          >  1:824985/1‑139 (MQ=255)   tCTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTc                                                                                                                        >  1:493753/1‑139 (MQ=255)    cTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCt                                                                                                                       >  2:892353/1‑139 (MQ=255)       aCCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACTTTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGaa                                                                                                                    <  2:645273/139‑1 (MQ=255)             ttGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAAt                                                                                                              >  2:8133/1‑139 (MQ=255)              tGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAAtt                                                                                                             >  1:447884/1‑139 (MQ=255)                     acGTCTGGCAGATATTCTCCAGGTTACATTCCCGTACCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCg                                                                                                      <  2:824985/139‑1 (MQ=255)                      cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                     >  2:937236/1‑139 (MQ=255)                                atatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                           <  1:892353/139‑1 (MQ=255)                                atatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                           <  2:108554/139‑1 (MQ=255)                                      tcCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCt                                                                                     <  1:185176/139‑1 (MQ=255)                                          ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                 <  1:662279/139‑1 (MQ=255)                                          ggTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga                                                                                 <  2:493753/139‑1 (MQ=255)                                               cATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAg                                                                            >  1:450719/1‑139 (MQ=255)                                                      gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                     <  1:531932/107‑1 (MQ=255)                                                      gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                     >  2:531932/1‑107 (MQ=255)                                                        gccgccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAgg                                                                   <  2:937873/139‑1 (MQ=255)                                                            ccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTggg                                                               >  1:994133/1‑139 (MQ=255)                                                              aTCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                     <  1:275064/99‑1 (MQ=255)                                                              aTCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCgg                                                                                                     >  2:275064/1‑99 (MQ=255)                                                                           cactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGc                                                                                       <  1:491963/100‑1 (MQ=255)                                                                           cactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGc                                                                                       >  2:491963/1‑100 (MQ=255)                                                                             ctGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                              <  2:322793/139‑1 (MQ=255)                                                                                aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                        >  2:187615/1‑78 (MQ=255)                                                                                aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                        <  1:187615/78‑1 (MQ=255)                                                                                aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCg                                           >  1:582131/1‑139 (MQ=255)                                                                                 tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                          <  2:447884/139‑1 (MQ=255)                                                                                 tCCGCCAATCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                           >  1:164114/1‑90 (MQ=255)                                                                                 tCCGCCAATCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaataa                                                                                           <  2:164114/90‑1 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGAt                                                             >  1:818763/1‑119 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGAt                                                             <  2:818763/119‑1 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                              >  1:135357/1‑134 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                              <  2:135357/134‑1 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                         <  1:137173/139‑1 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                         <  1:8133/139‑1 (MQ=255)                                                                                  ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTATCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCGGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                         <  2:994133/139‑1 (MQ=255)                                                                                         tCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGt                                  >  2:136219/1‑139 (MQ=255)                                                                                          cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAgg                                                                   <  2:568774/105‑1 (MQ=255)                                                                                          cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAgg                                                                   >  1:568774/1‑105 (MQ=255)                                                                                                          aCCTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACg                 >  2:513571/1‑139 (MQ=255)                                                                                                           ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGaa                                                                                                   <  2:391045/56‑1 (MQ=255)                                                                                                           ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGaa                                                                                                   >  1:391045/1‑56 (MQ=255)                                                                                                           ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAgg                                                                   <  2:944561/88‑1 (MQ=255)                                                                                                           ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAgg                                                                   >  1:944561/1‑88 (MQ=255)                                                                                                           ccTTCAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGc                <  1:1014276/139‑1 (MQ=255)                                                                                                               cAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                        >  2:345351/1‑47 (MQ=255)                                                                                                               cAGCAACGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGt                                                                                                        <  1:345351/47‑1 (MQ=255)                                                                                                                    aCGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaa                                                                                              >  1:649455/1‑52 (MQ=255)                                                                                                                    aCGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCaa                                                                                              <  2:649455/52‑1 (MQ=255)                                                                                                                    aCGGCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGcc       <  2:77423/139‑1 (MQ=255)                                                                                                                      ggCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCg                                                           <  2:7538/85‑1 (MQ=255)                                                                                                                      ggCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCg                                                           >  1:7538/1‑85 (MQ=255)                                                                                                                      ggCATGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccac     >  1:917908/1‑139 (MQ=255)                                                                                                                         aTGCTGCAGCTTGTTGGTTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCAt  <  1:513571/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCAT  >  NC_000913/4302104‑4302362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑