Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,352,880	G→A	*99* (TAG→TAA)	ghoS →	antitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,352,880	0	G	A	100.0%	152.0 / NA	48	*99* (TAG→TAA)	ghoS	antitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/28);  total (20/28)

TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACT  >  NC_000913/4352746‑4353011                                                                                                                                       |                                                                                                                                    ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCag                                                                                                                                 <  1:384515/139‑1 (MQ=255)  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                                <  2:112015/139‑1 (MQ=255)  tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                                <  1:898160/139‑1 (MQ=255)        tAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGAt                                                                                                                          <  1:664763/139‑1 (MQ=255)                  gTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACa                                                                                                                >  2:642867/1‑139 (MQ=255)                  gTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACa                                                                                                                >  2:981860/1‑139 (MQ=255)                        cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaa                                                                                                          >  1:166735/1‑139 (MQ=255)                           aaGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATg                                                                                                       >  2:142348/1‑139 (MQ=255)                            aGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATgg                                                                                                      <  1:142348/139‑1 (MQ=255)                              tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  2:269120/139‑1 (MQ=255)                              tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  1:240689/139‑1 (MQ=255)                              tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTAATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  1:725820/139‑1 (MQ=255)                                 aaTCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                                 <  2:814904/139‑1 (MQ=255)                                                    cTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                               <  1:523491/90‑1 (MQ=255)                                                    cTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                               >  2:523491/1‑90 (MQ=255)                                                    cTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                               >  1:246866/1‑90 (MQ=255)                                                    cTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                               <  2:246866/90‑1 (MQ=255)                                                             ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                     <  2:1808/139‑1 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATaagaa                                                                                                                     <  2:755285/83‑1 (MQ=255)                                                                     aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATaagaa                                                                                                                     >  1:755285/1‑83 (MQ=255)                                                                          atatTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACat                                                        <  2:76406/139‑1 (MQ=255)                                                                           tatTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACata                                                       <  1:878555/139‑1 (MQ=255)                                                                                 ttttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                         >  2:243908/1‑83 (MQ=255)                                                                                 ttttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                         <  1:243908/83‑1 (MQ=255)                                                                                     gACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCa                                             >  1:401536/1‑139 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                    >  1:918389/1‑100 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                    <  2:918389/100‑1 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                               >  1:844038/1‑105 (MQ=255)                                                                                                     cAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                               <  2:844038/105‑1 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                         >  1:754907/1‑60 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                         <  2:754907/60‑1 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                          >  1:851979/1‑139 (MQ=255)                                                                                                        ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                          >  1:206039/1‑139 (MQ=255)                                                                                                          gatgaCTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATg                        >  1:515267/1‑139 (MQ=255)                                                                                                             gaCTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATgaga                     <  1:449431/139‑1 (MQ=255)                                                                                                                 aTTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAac                 <  2:515267/139‑1 (MQ=255)                                                                                                                  tttCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    >  2:515404/1‑55 (MQ=255)                                                                                                                  tttCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  1:515404/55‑1 (MQ=255)                                                                                                                      cGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  2:401536/139‑1 (MQ=255)                                                                                                                          aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                     >  1:583804/1‑78 (MQ=255)                                                                                                                          aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                     <  2:583804/78‑1 (MQ=255)                                                                                                                          aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCg        >  1:418303/1‑139 (MQ=255)                                                                                                                            ttttACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCAtt                                           <  1:983462/102‑1 (MQ=255)                                                                                                                            ttttACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCAtt                                           >  2:983462/1‑102 (MQ=255)                                                                                                                                aCAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt  <  1:642867/139‑1 (MQ=255)                                                                                                                                    gTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGt                                    <  2:654703/101‑1 (MQ=255)                                                                                                                                    gTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGt                                    <  2:812620/101‑1 (MQ=255)                                                                                                                                    gTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGt                                    >  1:812620/1‑101 (MQ=255)                                                                                                                                       |                                                                                                                                    TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACT  >  NC_000913/4352746‑4353011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑