Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,419,456 T→C N57S (AAC→AGC)  ulaG ← L‑ascorbate 6‑phosphate lactonase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,419,4560TC100.0% 152.2 / NA 43N57S (AAC→AGC) ulaGL‑ascorbate 6‑phosphate lactonase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

ACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCA  >  NC_000913/4419324‑4419585
                                                                                                                                    |                                                                                                                                 
aCAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGc                                                                                                                             <  1:515154/139‑1 (MQ=255)
   aaCGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCAc                                                                                                                          >  2:398070/1‑139 (MQ=255)
       gggTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTc                                                                                                                      <  2:429476/139‑1 (MQ=255)
         gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCg                                                                                                                    >  2:900469/1‑139 (MQ=255)
             tACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGAttt                                                                                                                <  1:900469/139‑1 (MQ=255)
                  aGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAAcc                                                                                                           >  1:510178/1‑139 (MQ=255)
                      ttGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGAt                                                                                                       >  1:23206/1‑139 (MQ=255)
                        ggCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATcc                                                                                                     <  2:510178/139‑1 (MQ=255)
                                ttttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCag                                                                                             >  2:475878/1‑139 (MQ=255)
                                 tttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            <  2:528843/139‑1 (MQ=255)
                                 tttttCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            <  1:475878/139‑1 (MQ=255)
                                                    gcTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                         <  1:765771/139‑1 (MQ=255)
                                                        cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAAtgtg                                                                     >  2:495892/1‑139 (MQ=255)
                                                        cATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAAtgtg                                                                     >  1:771272/1‑139 (MQ=255)
                                                           cTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagccaagc                                                                                       <  2:337641/118‑1 (MQ=255)
                                                           cTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagccaagc                                                                                       >  1:337641/1‑118 (MQ=255)
                                                           cTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCa                                                                  <  2:990782/139‑1 (MQ=255)
                                                              gTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGa                                                               <  1:398070/139‑1 (MQ=255)
                                                                   ccTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                          >  1:503809/1‑139 (MQ=255)
                                                                        ttCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGtt                                                     >  1:648345/1‑139 (MQ=255)
                                                                            ttAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTg                                                 >  1:781585/1‑139 (MQ=255)
                                                                                      aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                          >  1:181681/1‑120 (MQ=255)
                                                                                      aCCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGAc                                                          <  2:181681/120‑1 (MQ=255)
                                                                                        cGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                         <  1:755351/103‑1 (MQ=255)
                                                                                        cGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCaaa                                                                         >  2:755351/1‑103 (MQ=255)
                                                                                        cGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATttcttc                                     <  1:968460/139‑1 (MQ=255)
                                                                                             cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGAt                                                                                                                  <  2:837479/57‑1 (MQ=255)
                                                                                             cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGAt                                                                                                                  >  1:837479/1‑57 (MQ=255)
                                                                                             cTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCttcattca                                <  1:526474/139‑1 (MQ=255)
                                                                                              tttgtttgCCAGTGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCaa                               <  2:227115/139‑1 (MQ=255)
                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCa                                                                                                          >  1:746068/1‑52 (MQ=255)
                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCa                                                                                                          <  2:746068/52‑1 (MQ=255)
                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            >  1:198312/1‑66 (MQ=255)
                                                                                                          tGCCGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                                                                            <  2:198312/66‑1 (MQ=255)
                                                                                                            ccGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCaa                                                                                                             <  1:744216/47‑1 (MQ=255)
                                                                                                            ccGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCaa                                                                                                             >  2:744216/1‑47 (MQ=255)
                                                                                                            ccGCACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACt                 >  1:1060680/1‑139 (MQ=255)
                                                                                                               cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCg              >  2:982920/1‑139 (MQ=255)
                                                                                                               cACCAGAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCg              >  2:132377/1‑139 (MQ=255)
                                                                                                                    gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGaaa         >  2:704740/1‑139 (MQ=255)
                                                                                                                    gAAATCAACGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGaaa         <  2:648345/139‑1 (MQ=255)
                                                                                                                           aCGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCa  <  1:982920/139‑1 (MQ=255)
                                                                                                                           aCGCAAACGCTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCa  <  2:1060680/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                 
ACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCA  >  NC_000913/4419324‑4419585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: