Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,425,834 G→A *105* (TAG→TAA priB → primosomal protein N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,425,8340GA100.0% 93.7 / NA 30*105* (TAG→TAApriBprimosomal protein N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

TATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTA  >  NC_000913/4425714‑4425968
                                                                                                                        |                                                                                                                                      
tataACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTAttt                                                                                                                      >  2:552556/1‑139 (MQ=255)
tataACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTAttt                                                                                                                      >  2:502850/1‑139 (MQ=255)
        tCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCgtcgtc                                                                                                              >  1:805564/1‑139 (MQ=255)
          ggCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGc                                                                                                            >  2:64293/1‑139 (MQ=255)
             aGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAg                                                                                                         <  1:374444/139‑1 (MQ=255)
                                  ttcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcgtcgt                                                                                                               <  1:60468/112‑1 (MQ=255)
                                  ttcatttcatGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcgtcgt                                                                                                               >  2:60468/1‑112 (MQ=255)
                                           tGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCaa                                                                           >  2:76320/1‑139 (MQ=255)
                                                 aaGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     <  1:64293/139‑1 (MQ=255)
                                                  aGGTAAAGAACGGACTGAACAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCg                                                                    <  1:850193/139‑1 (MQ=255)
                                                       aaGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   >  1:519620/1‑135 (MQ=255)
                                                       aaGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   <  2:519620/135‑1 (MQ=255)
                                                                   aGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     <  1:816704/121‑1 (MQ=255)
                                                                   aGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATc                                                                     >  2:816704/1‑121 (MQ=255)
                                                                    gCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   <  2:66675/122‑1 (MQ=255)
                                                                    gCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGa                                                                   >  1:66675/1‑122 (MQ=255)
                                                                             ttttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAagag                                                                        <  1:883397/108‑1 (MQ=255)
                                                                             ttttGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAagag                                                                        >  2:883397/1‑108 (MQ=255)
                                                                                  cATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAc                                    <  2:805564/139‑1 (MQ=255)
                                                                                          gCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                                   <  1:552495/52‑1 (MQ=255)
                                                                                          gCAGATTGAATTGATAGATTCTGGAGACTAACCATATGGCACGTTATTTCcg                                                                                                                   >  2:552495/1‑52 (MQ=255)
                                                                                              attgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa                           <  1:342287/136‑1 (MQ=255)
                                                                                              attgaattgaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaa                           >  2:342287/1‑136 (MQ=255)
                                                                                                      gaTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGAt                <  1:76320/139‑1 (MQ=255)
                                                                                                       aTAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGAtt               >  1:676653/1‑139 (MQ=255)
                                                                                                        tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                   >  2:191954/1‑70 (MQ=255)
                                                                                                        tAGATTCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAg                                                                                   <  1:191954/70‑1 (MQ=255)
                                                                                                             tCTGGAGACTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCa         <  2:676653/139‑1 (MQ=255)
                                                                                                                    aCTAACCATATGTCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAAATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTa  <  1:492888/139‑1 (MQ=255)
                                                                                                                    aCTAACCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTa  >  2:380581/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                                      
TATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTA  >  NC_000913/4425714‑4425968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: