| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,464,629 | G→A | S27S (AGC→AGT) | treC ← | trehalose‑6‑P hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,464,629 | 0 | G | A | 100.0% | 114.6 / NA | 36 | S27S (AGC→AGT) | treC | trehalose‑6‑P hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/17); total (19/17) | |||||||||||
CGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGC > NC_000913/4464506‑4464745 | cGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTcc > 2:865487/1‑139 (MQ=255) tACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGa > 1:783933/1‑139 (MQ=255) tACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGa > 1:660103/1‑139 (MQ=255) aaCCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCtt < 1:814020/139‑1 (MQ=255) ttATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGAt < 1:305155/139‑1 (MQ=255) cTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa > 2:518127/1‑130 (MQ=255) cTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa < 1:518127/130‑1 (MQ=255) tAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGt > 1:383610/1‑139 (MQ=255) gTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc < 2:816293/139‑1 (MQ=255) aGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTgggg < 2:383610/139‑1 (MQ=255) aGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTgggg < 1:865487/139‑1 (MQ=255) aTCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGa > 1:35748/1‑139 (MQ=255) aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccac < 2:390261/103‑1 (MQ=255) aTAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccac > 1:390261/1‑103 (MQ=255) tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa > 1:818292/1‑75 (MQ=255) tAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATaa < 2:818292/75‑1 (MQ=255) tCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAAc < 2:440346/86‑1 (MQ=255) tCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAAc > 1:440346/1‑86 (MQ=255) tgtTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCt > 1:425732/1‑125 (MQ=255) tgtTGGATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCt < 2:425732/125‑1 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCgtg < 1:309/38‑1 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCgtg > 2:309/1‑38 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc > 1:376743/1‑90 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGccacc < 2:376743/90‑1 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt < 2:694490/109‑1 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt > 1:694490/1‑109 (MQ=255) ggATAACGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGgcgc > 1:856059/1‑139 (MQ=255) aaCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt > 2:865041/1‑105 (MQ=255) aaCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCAt < 1:865041/105‑1 (MQ=255) aCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGaaa > 2:85308/1‑44 (MQ=255) aCGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGaaa < 1:85308/44‑1 (MQ=255) cGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa < 1:126739/53‑1 (MQ=255) cGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGa > 2:126739/1‑53 (MQ=255) cGCCACGTAAATCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTgc > 2:602764/1‑139 (MQ=255) tCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGAt < 2:314540/85‑1 (MQ=255) tCGCCGGTACCACTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGAt > 1:314540/1‑85 (MQ=255) | CGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTACCCGTGGTGTCCTGAAAACTCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGC > NC_000913/4464506‑4464745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |