Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,515,284 C→T G465D (GGC→GAC)  fecA ← TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,515,2840CT100.0% 256.7 / NA 79G465D (GGC→GAC) fecATonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/38);  total (41/38)

GTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTC  >  NC_000913/4515151‑4515417
                                                                                                                                     |                                                                                                                                     
gttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGAc                                                                                                                                  >  2:38960/1‑139 (MQ=255)
gttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGAc                                                                                                                                  >  2:802162/1‑139 (MQ=255)
 ttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACg                                                                                                                                 >  1:149054/1‑139 (MQ=255)
     ggTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCaa                                                                                                                             <  1:426748/139‑1 (MQ=255)
     ggTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCaa                                                                                                                             >  2:704427/1‑139 (MQ=255)
          gACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGcc                                                                                                                        <  1:704363/139‑1 (MQ=255)
           aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       >  2:1076509/1‑139 (MQ=255)
           aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       >  1:803207/1‑139 (MQ=255)
           aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       >  2:134401/1‑139 (MQ=255)
                            cGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCaa                                                                                                                             >  1:519795/1‑116 (MQ=255)
                            cGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCaa                                                                                                                             <  2:519795/116‑1 (MQ=255)
                               aTACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCgctg                                                                                                                   >  1:159845/1‑123 (MQ=255)
                               aTACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCgctg                                                                                                                   <  2:159845/123‑1 (MQ=255)
                               aTACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            <  1:1041822/130‑1 (MQ=255)
                               aTACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            >  2:1041822/1‑130 (MQ=255)
                                       cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAtt                                                                                           >  2:477387/1‑139 (MQ=255)
                                       cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAtt                                                                                           >  2:812657/1‑139 (MQ=255)
                                         tGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc                                                                                         <  1:134401/139‑1 (MQ=255)
                                           aTGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCAt                                                                                       >  1:341821/1‑139 (MQ=255)
                                           aTGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCAt                                                                                       >  1:959510/1‑139 (MQ=255)
                                             ggTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGc                                                                                     >  1:1025858/1‑139 (MQ=255)
                                                     tGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattc                                                                             >  2:11032/1‑139 (MQ=255)
                                                         gATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattcattc                                                                         >  2:747584/1‑139 (MQ=255)
                                                          aTGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGAttcattca                                                                        <  2:1025858/139‑1 (MQ=255)
                                                                 ttttGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCg                                                                 >  2:669184/1‑139 (MQ=255)
                                                                       cATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                           <  1:373682/139‑1 (MQ=255)
                                                                       cATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                           <  1:421849/139‑1 (MQ=255)
                                                                       cATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                           <  2:341821/139‑1 (MQ=255)
                                                                                aCCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTaa                                                                                                 <  1:695327/92‑1 (MQ=255)
                                                                                aCCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTaa                                                                                                 >  2:695327/1‑92 (MQ=255)
                                                                                aCCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa                                                                      <  2:429088/119‑1 (MQ=255)
                                                                                aCCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa                                                                      >  1:429088/1‑119 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtgg                                                                                                               <  2:382103/74‑1 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtgg                                                                                                               >  1:382103/1‑74 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCa                                                                                        <  2:475400/97‑1 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCa                                                                                        >  1:475400/1‑97 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgccca                                                         <  2:615812/128‑1 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgccca                                                         >  1:615812/1‑128 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg                                              >  1:558776/1‑139 (MQ=255)
                                                                                    ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCg                                              >  2:876199/1‑139 (MQ=255)
                                                                                     gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                             >  1:851746/1‑139 (MQ=255)
                                                                                      cGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            >  1:376961/1‑75 (MQ=255)
                                                                                      cGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            <  2:376961/75‑1 (MQ=255)
                                                                                      cGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTg                                            <  1:38960/139‑1 (MQ=255)
                                                                                        tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            <  1:759886/73‑1 (MQ=255)
                                                                                        tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGa                                                                >  2:336128/1‑117 (MQ=255)
                                                                                        tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGa                                                                <  1:336128/117‑1 (MQ=255)
                                                                                        tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg                                          <  2:149054/139‑1 (MQ=255)
                                                                                        tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGCAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg                                          >  1:88178/1‑139 (MQ=255)
                                                                                        tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAATCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            >  2:759886/1‑73 (MQ=255)
                                                                                         gcgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                         >  1:689243/1‑75 (MQ=255)
                                                                                         gcgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                         <  2:689243/75‑1 (MQ=255)
                                                                                          cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGcgcg                                        >  2:449551/1‑139 (MQ=255)
                                                                                                 gTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGCAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                                 <  2:88178/139‑1 (MQ=255)
                                                                                                  tGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            >  1:818835/1‑63 (MQ=255)
                                                                                                  tGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            <  2:818835/63‑1 (MQ=255)
                                                                                                  tGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                                <  2:851746/139‑1 (MQ=255)
                                                                                                   gCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                               <  1:477387/139‑1 (MQ=255)
                                                                                                            cgTATCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAg                      >  1:128340/1‑139 (MQ=255)
                                                                                                               aTCGCGGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATc                   <  2:959510/139‑1 (MQ=255)
                                                                                                                   cgGTCGTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGc               <  1:1009302/139‑1 (MQ=255)
                                                                                                                        gTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAg          <  1:1076509/139‑1 (MQ=255)
                                                                                                                        gTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAg          >  1:852188/1‑139 (MQ=255)
                                                                                                                        gTAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAg          >  1:810146/1‑139 (MQ=255)
                                                                                                                         tAAGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGAGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGc         >  2:803069/1‑139 (MQ=255)
                                                                                                                          aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg        <  2:494025/139‑1 (MQ=255)
                                                                                                                          aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg        <  2:852188/139‑1 (MQ=255)
                                                                                                                          aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg        <  2:810146/139‑1 (MQ=255)
                                                                                                                          aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg        <  1:802162/139‑1 (MQ=255)
                                                                                                                          aaGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg        <  2:558776/139‑1 (MQ=255)
                                                                                                                               cTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCt   <  1:747584/139‑1 (MQ=255)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCg                                                                                                          <  2:734490/35‑1 (MQ=38)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCg                                                                                                          >  1:734490/1‑35 (MQ=38)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACTCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTc  >  1:359740/1‑139 (MQ=255)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGTCCACTTCGTGCGCGGAAGGGcc                                >  2:1014008/1‑109 (MQ=255)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGTCCACTTCGTGCGCGGAAGGGcc                                <  1:1014008/109‑1 (MQ=255)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                               >  2:1008385/1‑110 (MQ=255)
                                                                                                                                ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                               <  1:1008385/110‑1 (MQ=255)
                                                                                                                                  gAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                           <  2:830399/80‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTC  >  NC_000913/4515151‑4515417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑