Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,577,958	C→T	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC ← mcrB ←	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,577,958	0	C	T	100.0%	230.4 / NA	71	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC mcrB	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/33);  total (38/33)

TACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577828‑4578091                                                                                                                                   |                                                                                                                                      tacCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                               <  1:571537/139‑1 (MQ=255)  acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  2:994956/1‑139 (MQ=255)  acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  2:706051/1‑139 (MQ=255)     ccAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCt                                                                                                                           >  1:274426/1‑139 (MQ=255)       aGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTATAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaa                                                                                                                         >  2:943712/1‑139 (MQ=255)           tatCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTAt                                                                                                                                    <  2:453499/124‑1 (MQ=255)           tatCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTAt                                                                                                                                    >  1:453499/1‑124 (MQ=255)            atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                               >  2:356900/1‑128 (MQ=255)            atCAAGAAGATTGTTACCGGGTATGGCTTCAAGGGTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTc                                                                                                                               <  1:356900/128‑1 (MQ=255)              cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                  >  2:554984/1‑139 (MQ=255)               aagaagATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  1:994956/139‑1 (MQ=255)               aagaagATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  1:199328/139‑1 (MQ=255)                    gaTTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGCTGGTc                                                                                                            >  1:668562/1‑139 (MQ=255)                       tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                         >  2:614719/1‑139 (MQ=255)                         ttACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       >  2:28368/1‑139 (MQ=255)                           aCCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     <  2:274426/139‑1 (MQ=255)                              gggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  2:668562/139‑1 (MQ=255)                                gTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgttgt                                                                                                <  1:28368/139‑1 (MQ=255)                                 tATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  >  2:944642/1‑136 (MQ=255)                                 tATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:944642/136‑1 (MQ=255)                                         cAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGt                                                                                                                                >  1:299460/1‑98 (MQ=255)                                         cAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGt                                                                                                                                <  2:299460/98‑1 (MQ=255)                                               ttGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 <  1:614719/139‑1 (MQ=255)                                                      cTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 >  1:20693/1‑100 (MQ=255)                                                      cTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  2:20693/100‑1 (MQ=255)                                                             tCCTATAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttcttc                                                                   <  1:943712/139‑1 (MQ=255)                                                                        aCCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                        <  1:184633/139‑1 (MQ=255)                                                                                     gTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:340376/53‑1 (MQ=255)                                                                                     gTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  2:340376/1‑53 (MQ=255)                                                                                          cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACaa                                      >  2:1031272/1‑139 (MQ=255)                                                                                          cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACaa                                      >  2:364487/1‑139 (MQ=255)                                                                                           aTGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAt                                     <  1:147389/139‑1 (MQ=255)                                                                                               aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  >  1:1065061/1‑74 (MQ=255)                                                                                               aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  2:1065061/74‑1 (MQ=255)                                                                                               aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCa                                 >  1:812787/1‑139 (MQ=255)                                                                                               aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCa                                 >  2:919719/1‑139 (MQ=255)                                                                                                aTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCAt                                >  2:816436/1‑139 (MQ=255)                                                                                                      atTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:152727/36‑1 (MQ=255)                                                                                                      atTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  2:152727/1‑36 (MQ=255)                                                                                                      atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGcc                          >  2:334138/1‑139 (MQ=255)                                                                                                       ttACGGACAGGTATCTCGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAGATATTCTTCGAGTAAAGGGGCGata                                                   >  2:716670/1‑113 (MQ=255)                                                                                                       ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGata                                                   <  1:716670/113‑1 (MQ=255)                                                                                                       ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCa                         >  2:57319/1‑139 (MQ=255)                                                                                                        tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  1:480483/55‑1 (MQ=255)                                                                                                        tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            >  2:480483/1‑55 (MQ=255)                                                                                                        tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGaaa                                                                           <  1:314676/88‑1 (MQ=255)                                                                                                        tACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGaaa                                                                           >  2:314676/1‑88 (MQ=255)                                                                                                         aCGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   <  1:601796/79‑1 (MQ=255)                                                                                                         aCGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   >  2:601796/1‑79 (MQ=255)                                                                                                          cGGACAGGTATCACGGGCTGTTCCATTATGAGTccc                                                                                                                             <  1:798784/36‑1 (MQ=255)                                                                                                          cGGACAGGTATCACGGGCTGTTCCATTATGAGTccc                                                                                                                             >  2:798784/1‑36 (MQ=255)                                                                                                          cGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTg                      <  1:706051/139‑1 (MQ=255)                                                                                                           ggACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                        >  1:890673/1‑104 (MQ=255)                                                                                                           ggACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                        <  2:890673/104‑1 (MQ=255)                                                                                                               aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTa                                                              >  1:74091/1‑94 (MQ=255)                                                                                                               aGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTa                                                              <  2:74091/94‑1 (MQ=255)                                                                                                                     cACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGgag           <  1:57319/139‑1 (MQ=255)                                                                                                                      aCGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 >  2:46253/1‑36 (MQ=255)                                                                                                                      aCGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  1:46253/36‑1 (MQ=255)                                                                                                                         ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                    <  2:589949/46‑1 (MQ=255)                                                                                                                         ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                    >  1:589949/1‑46 (MQ=255)                                                                                                                           cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATcc                                                >  2:798223/1‑96 (MQ=255)                                                                                                                           cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATcc                                                <  1:798223/96‑1 (MQ=255)                                                                                                                            tGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCACCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGcc    >  1:55066/1‑139 (MQ=255)                                                                                                                             gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCa   <  2:812787/139‑1 (MQ=255)                                                                                                                              ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                    >  2:859246/1‑41 (MQ=255)                                                                                                                              ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtg                                                                                                    <  1:859246/41‑1 (MQ=255)                                                                                                                              ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttct                                                                     >  2:247125/1‑72 (MQ=255)                                                                                                                              ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATAttct                                                                     <  1:247125/72‑1 (MQ=255)                                                                                                                              ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  1:738865/1‑139 (MQ=255)                                                                                                                              ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  1:581428/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                      TACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577828‑4578091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑