| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 443,604 | C→T | S13N (AGT→AAT) *304* (TAG→TAA) |
yajL ← panE ← |
oxidative‑stress‑resistance chaperone 2‑dehydropantoate reductase, NADPH‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 443,604 | 0 | C | T | 97.2% | 105.3 / ‑4.0 | 36 | S13N (AGT→AAT) *304* (TAG→TAA) | yajL panE | oxidative‑stress‑resistance chaperone 2‑dehydropantoate reductase, NADPH‑specific |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (18/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAC > NC_000913/443485‑443731 | gcTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAAc < 1:57804/139‑1 (MQ=255) cgcgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGa < 1:561841/139‑1 (MQ=255) gcgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGAt < 2:685945/139‑1 (MQ=255) cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg < 1:198780/139‑1 (MQ=255) cgcgAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg < 2:147258/139‑1 (MQ=255) cAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCa < 2:164497/139‑1 (MQ=255) aGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg > 2:454586/1‑121 (MQ=255) aGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg < 1:454586/121‑1 (MQ=255) gTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCctt > 1:393899/1‑139 (MQ=255) cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg > 1:551435/1‑111 (MQ=255) cTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATgcg < 2:551435/111‑1 (MQ=255) cGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCAttt > 2:459471/1‑139 (MQ=255) gCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCaaa > 2:268676/1‑139 (MQ=255) tggtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct < 1:393036/112‑1 (MQ=255) tggtgACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct > 2:393036/1‑112 (MQ=255) tttGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTc > 2:281999/1‑139 (MQ=255) gATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGTCGATGCGCTCATATTc < 2:321669/94‑1 (MQ=255) gATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGTCGATGCGCTCATATTc > 1:321669/1‑94 (MQ=255) gATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCgg > 2:751463/1‑139 (MQ=255) aTACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGt > 1:759655/1‑139 (MQ=255) gccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGc < 1:268676/139‑1 (MQ=255) ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg < 2:964440/76‑1 (MQ=255) ccgcGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATg > 1:964440/1‑76 (MQ=255) aaCCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCGCTCTCCTTTCTATTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATccc < 2:759655/139‑1 (MQ=255) aTAGTGGTGACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg < 1:751463/139‑1 (MQ=255) gtggtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATg < 2:199908/126‑1 (MQ=255) gtggtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATg > 1:199908/1‑126 (MQ=255) gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct > 2:1058515/1‑71 (MQ=255) gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCct < 1:1058515/71‑1 (MQ=255) gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg < 2:686630/133‑1 (MQ=255) gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCgcgcgggcg > 1:686630/1‑133 (MQ=255) gtgACGGCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGt > 2:185209/1‑139 (MQ=255) ggCTTCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAagag > 2:1002123/1‑139 (MQ=255) tCAGTCTCTTCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTC‑TTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAc > 1:1061505/1‑139 (MQ=255) ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa < 2:499437/129‑1 (MQ=255) ttCATTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa > 1:499437/1‑129 (MQ=255) | GCTTCACGCCGCGCGAGCAGGTAATCGCCAGGTTACCATCGCTGGCGACGCTGGCAGTGGTGACTTTGATACCGCCGCGAACCAGCAGATCGATAGTGGTGACGGCTTCAGTCTCTTCACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAC > NC_000913/443485‑443731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |