Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,510	A→G	E43G (GAG→GGG)	ybaO →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,510	0	A	G	100.0%	121.9 / NA	35	E43G (GAG→GGG)	ybaO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

AAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGG  >  NC_000913/468376‑468638                                                                                                                                       |                                                                                                                                 aaGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgac                                                                                                                              <  1:858737/139‑1 (MQ=255)     gCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                          <  2:215689/139‑1 (MQ=255)        aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                            >  2:426014/1‑134 (MQ=255)        aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                            <  1:426014/134‑1 (MQ=255)        aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                                       >  1:157027/1‑139 (MQ=255)             aGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCtt                                                                                                                  <  2:561441/139‑1 (MQ=255)                   aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGc                                                                                                            >  2:607837/1‑139 (MQ=255)                        aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGt                                                                                                       >  2:293319/1‑139 (MQ=255)                         ccGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTc                                                                                                      >  2:360826/1‑139 (MQ=255)                         ccGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTc                                                                                                      >  2:724897/1‑139 (MQ=255)                         ccGTAAGCTGCTGGCCTTACTGCAGCAGGACTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTc                                                                                                      >  2:32917/1‑139 (MQ=255)                          cGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                     <  1:607837/139‑1 (MQ=255)                           gTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGc                                                                                                    <  1:293319/139‑1 (MQ=255)                                        cTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                          <  1:600800/104‑1 (MQ=255)                                        cTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                          >  2:600800/1‑104 (MQ=255)                                          tACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGa                                                                                     >  1:505277/1‑139 (MQ=255)                                                 gcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATa                                                                              >  2:563142/1‑139 (MQ=255)                                                    ggATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGc                                                                           <  2:505277/139‑1 (MQ=255)                                                            cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCt                                                                   <  2:239189/139‑1 (MQ=255)                                                               tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                                <  2:157027/139‑1 (MQ=255)                                                               tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                                <  1:360826/139‑1 (MQ=255)                                                                         ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtgtg                                                      <  1:563142/139‑1 (MQ=255)                                                                           cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                                          >  1:926286/1‑101 (MQ=255)                                                                           cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGAt                                                                                          <  2:926286/101‑1 (MQ=255)                                                                           cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt                                                    >  1:611957/1‑139 (MQ=255)                                                                           cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt                                                    >  1:398432/1‑139 (MQ=255)                                                                                gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                     <  2:1043655/85‑1 (MQ=255)                                                                                gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                     >  1:1043655/1‑85 (MQ=255)                                                                                          ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACa                                     >  2:94368/1‑139 (MQ=255)                                                                                                  aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcagca                             >  1:766947/1‑139 (MQ=255)                                                                                                      ccaccCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGa                         <  1:367167/139‑1 (MQ=255)                                                                                                                 tGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                     <  1:833429/52‑1 (MQ=255)                                                                                                                 tGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                     >  2:833429/1‑52 (MQ=255)                                                                                                                             aaaCGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACgg  <  2:1028049/139‑1 (MQ=255)                                                                                                                                    tGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCAc    <  1:36600/130‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 AAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGG  >  NC_000913/468376‑468638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑