Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,139,406 Δ1 bp coding (1029/1644 nt) flgK → flagellar hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,139,4060T.100.0% 199.7 / NA 47coding (1029/1644 nt)flgKflagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/25);  total (22/25)

GACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGA  >  NC_000913/1139273‑1139530
                                                                                                                                     |                                                                                                                            
gACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGtt                                                                                                                        >  2:58632/1‑139 (MQ=255)
   aaCTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCg                                                                                                                     <  1:623849/139‑1 (MQ=255)
             ggCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCa                                                                                                           <  1:1177630/139‑1 (MQ=255)
                aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACgg                                                                                                        <  1:58632/139‑1 (MQ=255)
                        aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                >  1:625435/1‑139 (MQ=255)
                                    cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATc                                                                                                                  >  2:623130/1‑109 (MQ=255)
                                    cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATc                                                                                                                  <  1:623130/109‑1 (MQ=255)
                                    cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                    <  1:81882/139‑1 (MQ=255)
                                       aaCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACTCGAAAAACAAAGGGACGTTGTGAt                                                                                                                   <  2:911104/105‑1 (MQ=255)
                                       aaCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGTGAt                                                                                                                   >  1:911104/1‑105 (MQ=255)
                                           caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                             <  1:407283/139‑1 (MQ=255)
                                            aaaGCCGGATTCGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGAc                                                                            <  2:735677/139‑1 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCa                                                                                                           <  1:706550/106‑1 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCa                                                                                                           >  2:706550/1‑106 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcgcg                                                                                       <  2:565740/126‑1 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         >  2:1232189/1‑124 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         <  1:1232189/124‑1 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                          >  1:244962/1‑139 (MQ=255)
                                              aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGACTCcgcg                                                                                       >  1:565740/1‑126 (MQ=255)
                                                        gATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAAt                                                                >  1:349032/1‑139 (MQ=255)
                                                          tGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAAtct                                                              >  2:70797/1‑139 (MQ=255)
                                                            cTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCg                                                            <  2:381307/139‑1 (MQ=255)
                                                              aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                         <  1:381791/124‑1 (MQ=255)
                                                              aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                         >  2:381791/1‑124 (MQ=255)
                                                                           gTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAg                                             <  2:349032/139‑1 (MQ=255)
                                                                            tGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGt                                            <  2:244962/139‑1 (MQ=255)
                                                                                     cTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCa                                   <  2:625435/139‑1 (MQ=255)
                                                                                      tttGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  2:1138341/115‑1 (MQ=255)
                                                                                      tttGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          >  1:1138341/1‑115 (MQ=255)
                                                                                             tCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                           <  1:70797/139‑1 (MQ=255)
                                                                                             tCGGCAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                    <  1:267394/82‑1 (MQ=255)
                                                                                             tCGGCAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                    >  2:267394/1‑82 (MQ=255)
                                                                                               ggTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCg                                                                                                                     >  2:930546/1‑47 (MQ=255)
                                                                                               ggTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCg                                                                                                                     <  1:930546/47‑1 (MQ=255)
                                                                                                gTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGcc                        >  2:443864/1‑139 (MQ=255)
                                                                                                         cgGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAAt                  <  2:1183530/136‑1 (MQ=255)
                                                                                                         cgGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAAt                  >  1:1183530/1‑136 (MQ=255)
                                                                                                          ggTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         >  1:920677/1‑64 (MQ=255)
                                                                                                          ggTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         <  2:920677/64‑1 (MQ=255)
                                                                                                               tGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTc                                                                                           >  2:1296585/1‑57 (MQ=255)
                                                                                                               tGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTc                                                                                           <  1:1296585/57‑1 (MQ=255)
                                                                                                                      cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          >  2:506836/1‑83 (MQ=255)
                                                                                                                      cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  1:506836/83‑1 (MQ=255)
                                                                                                                      cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa  >  2:556674/1‑139 (MQ=255)
                                                                                                                      cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa  >  1:157252/1‑139 (MQ=255)
                                                                                                                           aaaaCAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAg                      <  2:33142/114‑1 (MQ=255)
                                                                                                                           aaaaCAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAg                      >  1:33142/1‑114 (MQ=255)
                                                                                                                                     |                                                                                                                            
GACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGA  >  NC_000913/1139273‑1139530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: