Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5370C.100.0% 107.1 / NA 26coding (1052/1404 nt)fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (11/15);  total (11/15)

GCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTG  >  NC_000913/1685400‑1685633
                                                                                                                                         |                                                                                                
gCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                 <  1:1232967/139‑1 (MQ=255)
              tCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGc                                                                                  <  1:478991/139‑1 (MQ=255)
              tCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAAGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGc                                                                                  <  2:771720/139‑1 (MQ=255)
                aaTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                <  1:73262/139‑1 (MQ=255)
                          gcgcAGTGTTTGTTAAAACTTTCCATGCTATCTGACAGCAAGCGCACCGCTTGCAGGAAATAGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                      >  2:869925/1‑139 (MQ=255)
                          gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCTCCGATTGCAGGAAATTGCGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                      <  2:866109/139‑1 (MQ=255)
                           cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccc                                                                                                <  2:509695/113‑1 (MQ=255)
                           cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccc                                                                                                >  1:509695/1‑113 (MQ=255)
                             cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                   <  2:319676/139‑1 (MQ=255)
                                          aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcg                                                                              <  1:1136840/115‑1 (MQ=255)
                                          aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcg                                                                              >  2:1136840/1‑115 (MQ=255)
                                                         cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                       >  2:848694/1‑139 (MQ=255)
                                                              aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                  <  1:848694/139‑1 (MQ=255)
                                                                cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                                >  1:544838/1‑139 (MQ=255)
                                                                             tGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                 >  1:229705/1‑93 (MQ=255)
                                                                             tGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                 <  2:229705/93‑1 (MQ=255)
                                                                                 ggAAATTGTGGATCACCATTGGACGTAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAGGCCTCACACTGTGTTGGGTTCACTTTc               <  1:869925/139‑1 (MQ=255)
                                                                                 ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGc                                                                                  >  1:626933/1‑72 (MQ=255)
                                                                                 ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGc                                                                                  <  2:626933/72‑1 (MQ=255)
                                                                                 ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc               <  1:661666/139‑1 (MQ=255)
                                                                                 ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc               <  1:96831/139‑1 (MQ=255)
                                                                                            aTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                        >  1:571518/1‑87 (MQ=255)
                                                                                            aTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc                                                        <  2:571518/87‑1 (MQ=255)
                                                                                              cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTg  >  2:476364/1‑139 (MQ=255)
                                                                                              cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTg  >  1:395334/1‑139 (MQ=255)
                                                                                                        cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                 >  2:22256/1‑35 (MQ=255)
                                                                                                        cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                 <  1:22256/35‑1 (MQ=255)
                                                                                                                     gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                       >  2:1098912/1‑79 (MQ=255)
                                                                                                                     gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                       <  1:1098912/79‑1 (MQ=255)
                                                                                                                           aGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                      >  2:583668/1‑42 (MQ=38)
                                                                                                                           aGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                      <  1:583668/42‑1 (MQ=255)
                                                                                                                                         |                                                                                                
GCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTG  >  NC_000913/1685400‑1685633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: