Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 125.5 / NA 30coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/17);  total (13/17)

CACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAC  >  NC_000913/1874917‑1875168
                                                                                                                                      |                                                                                                                     
cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccc                                                                                                                   >  1:599866/1‑139 (MQ=255)
          tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAgg                                                                                                        >  2:1281381/1‑139 (MQ=255)
            aaGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGcc                                                                                                      <  2:470339/139‑1 (MQ=255)
               cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCtt                                                                                                   >  1:467789/1‑139 (MQ=255)
                    tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGcc                                                                                              <  2:599866/139‑1 (MQ=255)
                                aCTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTccacc                                                                                  <  1:1044344/139‑1 (MQ=255)
                                     tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           <  2:615251/125‑1 (MQ=255)
                                     tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCcatc                                                                                           >  1:615251/1‑125 (MQ=255)
                                      gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                            >  1:429668/1‑139 (MQ=255)
                                         gCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCgg                                                                         <  1:1227422/139‑1 (MQ=255)
                                                 cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                                                 <  1:1281381/139‑1 (MQ=255)
                                                                    ctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAgg                                              <  1:1140790/139‑1 (MQ=255)
                                                                                 tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGc                                                                                                       >  1:284599/1‑69 (MQ=255)
                                                                                 tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGc                                                                                                       <  2:284599/69‑1 (MQ=255)
                                                                                       aaGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                             <  1:742779/73‑1 (MQ=255)
                                                                                       aaGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCca                                                                                             >  2:742779/1‑73 (MQ=255)
                                                                                           ttGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg                                                   >  1:783397/1‑111 (MQ=255)
                                                                                           ttGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg                                                   <  2:783397/111‑1 (MQ=255)
                                                                                               gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGAt                                                               <  2:38086/95‑1 (MQ=255)
                                                                                               gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGAt                                                               >  1:38086/1‑95 (MQ=255)
                                                                                                   aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                              <  2:25043/124‑1 (MQ=255)
                                                                                                   aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                              >  1:25043/1‑124 (MQ=255)
                                                                                                       aTCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtcatc           >  1:73340/1‑139 (MQ=255)
                                                                                                         cGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACGGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaa         >  1:1279925/1‑139 (MQ=255)
                                                                                                          gCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaaa        <  2:429668/139‑1 (MQ=255)
                                                                                                            aaaaaaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACa      <  2:73340/139‑1 (MQ=255)
                                                                                                             aaaaaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaaa        >  2:1194481/1‑136 (MQ=255)
                                                                                                             aaaaaGCCAGCCCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaaa        <  1:1194481/136‑1 (MQ=255)
                                                                                                                aaGCCAGCCCATTGCCCCAATGCCCCCACTCGACGGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc  <  2:1279925/139‑1 (MQ=255)
                                                                                                                   ccagccCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc                            >  1:615617/1‑110 (MQ=255)
                                                                                                                   ccagccCATTGCCCCAATGCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc                            <  2:615617/110‑1 (MQ=255)
                                                                                                                                      |                                                                                                                     
CACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAC  >  NC_000913/1874917‑1875168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: