Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,029,729 (C)6→5 coding (191/921 nt) yedA → amino acid exporter for phenylalanine, threonine

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,029,7240C.100.0% 155.4 / NA 37coding (186/921 nt)yedAamino acid exporter for phenylalanine, threonine
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (17/20);  total (17/20)

ATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NC_000913/2029588‑2029858
                                                                                                                                        |                                                                                                                                      
atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTccc                                                                                                                                      <  2:254729/139‑1 (MQ=255)
       ttGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTAc                                                                                                                              <  2:854558/139‑1 (MQ=255)
         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAGAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGt                                                                                                                            >  1:899910/1‑139 (MQ=255)
         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTcccc                                                                                                                                     >  1:1222869/1‑131 (MQ=255)
         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTcccc                                                                                                                                     <  2:1222869/131‑1 (MQ=255)
         ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGt                                                                                                                            >  2:695886/1‑139 (MQ=255)
           ggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTcc                                                                                                                          >  2:116946/1‑139 (MQ=255)
            gCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCg                                                                                                                         <  1:1145193/139‑1 (MQ=255)
                      ttttGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATg                                                                                                               <  1:116946/139‑1 (MQ=255)
                           tCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCcgcg                                                                                                          <  1:170380/139‑1 (MQ=255)
                                cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     <  1:961509/139‑1 (MQ=255)
                                  gATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTg                                                                                                   <  1:835317/139‑1 (MQ=255)
                                       gCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTg                                                                                              <  2:539305/139‑1 (MQ=255)
                                                   ggCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggct                                                                                  <  1:56750/139‑1 (MQ=255)
                                                   ggCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggct                                                                                  >  2:56750/1‑139 (MQ=255)
                                                    gCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTc                                                                                                                           <  2:1313168/97‑1 (MQ=255)
                                                    gCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTc                                                                                                                           >  1:1313168/1‑97 (MQ=255)
                                                      cTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTc                                                                               >  2:480778/1‑139 (MQ=255)
                                                              atgatgGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATgg                                                                       >  2:934417/1‑139 (MQ=255)
                                                              atgatgGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATgg                                                                       >  1:906863/1‑139 (MQ=255)
                                                                   ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      >  1:319580/1‑103 (MQ=255)
                                                                   ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      <  2:319580/103‑1 (MQ=255)
                                                                        gcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTcccccg                                                                                                                                   <  1:85594/70‑2 (MQ=255)
                                                                        gcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTcccccg                                                                                                                                   >  2:85594/1‑69 (MQ=255)
                                                                              gATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGcc                                                       <  1:934417/139‑1 (MQ=255)
                                                                                ttCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGa                                                     <  2:906863/139‑1 (MQ=255)
                                                                                             gTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGAc                                                              <  2:420742/117‑1 (MQ=255)
                                                                                             gTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGAc                                                              >  1:420742/1‑117 (MQ=255)
                                                                                                          ggCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCaa                                              <  1:245134/120‑1 (MQ=255)
                                                                                                          ggCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCaa                                              >  2:245134/1‑120 (MQ=255)
                                                                                                          ggCATTTTTGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  1:1227200/1‑139 (MQ=255)
                                                                                                                  tGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  1:695886/139‑1 (MQ=255)
                                                                                                                  tGCTACTGCGCGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  2:1055960/139‑1 (MQ=255)
                                                                                                                         gcgcGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  2:269383/53‑1 (MQ=255)
                                                                                                                         gcgcGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  >  1:269383/1‑53 (MQ=255)
                                                                                                                         gcgcGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  2:374987/1‑67 (MQ=255)
                                                                                                                         gcgcGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    <  1:374987/67‑1 (MQ=255)
                                                                                                                         gcgcGGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTg            >  2:355479/1‑139 (MQ=255)
                                                                                                                          cgcgGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGcc                                                                                                <  2:1137246/54‑1 (MQ=255)
                                                                                                                          cgcgGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGcc                                                                                                >  1:1137246/1‑54 (MQ=255)
                                                                                                                          cgcgGACACAAACTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           >  1:41385/1‑139 (MQ=255)
                                                                                                                                   aaaCTCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:41385/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAACTCCCCCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NC_000913/2029588‑2029858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: