Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,200,4470A.100.0% 173.4 / NA 41coding (169/3633 nt)yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/20);  total (21/20)

ACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCT  >  NC_000913/2200310‑2200573
                                                                                                                                         |                                                                                                                              
aCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaagaa                                                                                                                               >  2:65959/1‑139 (MQ=255)
       aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                        >  2:488449/1‑137 (MQ=255)
                 aaaTATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCg                                                                                                             >  1:508354/1‑139 (MQ=255)
                     ataAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTAt                                                                                                         <  1:769633/139‑1 (MQ=255)
                                    cgccgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTg                                                                                          <  1:65959/139‑1 (MQ=255)
                                          tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                    <  1:173526/139‑1 (MQ=255)
                                               cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                               <  1:814477/139‑1 (MQ=255)
                                                   gtcgCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGcc                                                                           >  2:584969/1‑139 (MQ=255)
                                                                    ccAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCaa                                                          >  2:1281266/1‑139 (MQ=255)
                                                                             gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGATACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                 >  1:878869/1‑139 (MQ=255)
                                                                               tATCACTGGAAGTCTCATCTAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                               <  2:878869/139‑1 (MQ=255)
                                                                                               aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTTGCAGTc                                                                               <  1:161672/91‑1 (MQ=255)
                                                                                               aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                               >  2:161672/1‑91 (MQ=255)
                                                                                               aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                               >  2:374028/1‑139 (MQ=255)
                                                                                                     aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                         <  1:584969/139‑1 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                    >  1:580594/1‑92 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                    <  2:580594/92‑1 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                              <  2:936646/130‑1 (MQ=255)
                                                                                                         tGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                              >  1:936646/1‑130 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGcc                                                                           >  2:290046/1‑83 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGcc                                                                           <  1:290046/83‑1 (MQ=255)
                                                                                                           ggTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                   >  1:916012/1‑139 (MQ=255)
                                                                                                              gatgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAAt                >  1:36153/1‑139 (MQ=255)
                                                                                                                tgaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg              <  1:42657/139‑1 (MQ=255)
                                                                                                                 gaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTAt                                                                                                         <  1:907552/47‑1 (MQ=255)
                                                                                                                 gaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTAt                                                                                                         >  2:907552/1‑47 (MQ=255)
                                                                                                                 gaATTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGACCGCCATCACGCAGGCCATCAACATGATCGGCGAACACAAAACCTAAATCCCGGCCCGGACAAAggc             >  2:253905/1‑139 (MQ=255)
                                                                                                                   aTTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                   >  2:946922/1‑83 (MQ=255)
                                                                                                                   aTTGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                   <  1:946922/83‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg         >  2:262321/1‑139 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg         <  2:916012/139‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg         <  1:374028/139‑1 (MQ=255)
                                                                                                                     tGCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAACCCCGGCCCGGACAATGacggcg         <  1:253905/139‑1 (MQ=255)
                                                                                                                      gCTACAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGgcgc        >  2:187395/1‑139 (MQ=255)
                                                                                                                         aCAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                               <  2:635437/65‑1 (MQ=255)
                                                                                                                         aCAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                               >  1:635437/1‑65 (MQ=255)
                                                                                                                         aCAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                              >  1:674041/1‑114 (MQ=255)
                                                                                                                         aCAAAAAGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                              <  2:674041/114‑1 (MQ=255)
                                                                                                                            aaaaGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt  >  2:188862/1‑139 (MQ=255)
                                                                                                                               aGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                    <  2:572040/54‑1 (MQ=255)
                                                                                                                               aGCTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                    >  1:572040/1‑54 (MQ=255)
                                                                                                                                 cTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAaca                                           >  2:618898/1‑93 (MQ=255)
                                                                                                                                 cTGAGAAGAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAaca                                           <  1:618898/93‑1 (MQ=255)
                                                                                                                                         |                                                                                                                              
ACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCT  >  NC_000913/2200310‑2200573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: