Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,496 Δ1 bp coding (1081/1086 nt) mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,4960G.97.1% 133.2 / ‑1.7 34coding (1081/1086 nt)mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (13/20);  minor base T (0/1);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.34e-01

AAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAC  >  NC_000913/2824366‑2824599
                                                                                                                                  |                                                                                                       
aaaTTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgc                                                                                                <  2:670179/139‑1 (MQ=255)
             aCAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCt                                                                                   <  2:992944/139‑1 (MQ=255)
                      cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAAc                                                                          >  1:1210939/1‑139 (MQ=255)
                      cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAAc                                                                          >  1:170199/1‑139 (MQ=255)
                                 ggAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGc                                                               <  2:167652/139‑1 (MQ=255)
                                  gAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                             <  2:506370/124‑1 (MQ=255)
                                  gAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                             >  1:506370/1‑124 (MQ=255)
                                            ttGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAAt                                                    >  2:1078426/1‑139 (MQ=255)
                                             tGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGTTCACGCCCCCCTCTTGTAAATAGTTATTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATg                                                   <  2:930090/139‑1 (MQ=255)
                                               gCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                 >  1:303286/1‑139 (MQ=255)
                                                   tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                             <  1:354929/139‑1 (MQ=255)
                                                    gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCGCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     <  2:467699/98‑1 (MQ=255)
                                                    gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCGCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     >  1:467699/1‑98 (MQ=255)
                                                    gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                            <  2:1210939/139‑1 (MQ=255)
                                                    gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                            >  2:113004/1‑139 (MQ=255)
                                                      aaTTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtgtg                                          <  2:303286/139‑1 (MQ=255)
                                                            aCGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTa                                    <  2:683239/139‑1 (MQ=255)
                                                                  aggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                              <  1:113004/139‑1 (MQ=255)
                                                                    gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            <  1:616086/139‑1 (MQ=255)
                                                                    gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            >  2:616086/1‑139 (MQ=255)
                                                                    aaTTCAGAAAAAGGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            <  2:145998/138‑1 (MQ=255)
                                                                                     aTTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            <  1:428991/122‑1 (MQ=255)
                                                                                     aTTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            >  2:428991/1‑122 (MQ=255)
                                                                                      ttAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCa          <  1:1078426/139‑1 (MQ=255)
                                                                                         gCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTACTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc       <  2:1117526/139‑1 (MQ=255)
                                                                                            agagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCa          <  2:294778/133‑1 (MQ=255)
                                                                                            agagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCa          >  1:294778/1‑133 (MQ=255)
                                                                                              agGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc  <  2:170199/139‑1 (MQ=255)
                                                                                               gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                    >  2:1319740/1‑56 (MQ=255)
                                                                                               gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                    <  1:1319740/56‑1 (MQ=255)
                                                                                               gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            <  2:1256695/112‑1 (MQ=255)
                                                                                               gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            >  1:1256695/1‑112 (MQ=255)
                                                                                                        aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc  <  2:1294427/129‑1 (MQ=255)
                                                                                                        aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc  >  1:1294427/1‑129 (MQ=255)
                                                                                                                                  |                                                                                                       
AAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAC  >  NC_000913/2824366‑2824599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: