Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,006 (C)6→5 intergenic (‑82/+27) nlpI ← / ← pnp lipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,0010C.100.0% 146.0 / NA 35intergenic (‑77/+32)nlpI/pnplipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (18/17);  total (18/17)

CATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCA  >  NC_000913/3308869‑3309132
                                                                                                                                    |                                                                                                                                   
cATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccac                                                                                                                               >  1:1094445/1‑137 (MQ=255)
  tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGGTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccg                                                                                                                             >  2:5564/1‑135 (MQ=255)
    cTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgca                                                                                                                           <  2:1209597/139‑7 (MQ=255)
     tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTACTAACAAGGCGTCCTGCCCGGTTAAAAGcccccaccgcag                                                                                                                          >  2:846994/1‑132 (MQ=255)
           cGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAgg                                                                                                                   >  1:1095531/1‑139 (MQ=255)
                   cTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGAGGAGGGCAAATgg                                                                                                           <  2:588020/139‑1 (MQ=255)
                              aaaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTc                                                                                                <  1:5564/139‑1 (MQ=255)
                                       cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                           >  1:18031/1‑119 (MQ=255)
                                       cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATgg                                                                                                           <  2:18031/119‑1 (MQ=255)
                                       cgcAAAAAAGGCTTCATTTCCCACCCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                                       >  1:1002342/1‑139 (MQ=255)
                                           aaaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAAc                                                                                                       <  2:313267/119‑1 (MQ=255)
                                           aaaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAAc                                                                                                       >  1:313267/1‑119 (MQ=255)
                                                        ttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCg                                                                      <  1:423758/139‑1 (MQ=255)
                                                                cccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgca                                                              >  1:220536/1‑139 (MQ=255)
                                                                 ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                             <  2:1002342/139‑1 (MQ=255)
                                                                         cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                               >  1:971555/1‑113 (MQ=255)
                                                                         cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCg                                                                               <  2:971555/113‑1 (MQ=255)
                                                                         cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                                     >  1:902545/1‑139 (MQ=255)
                                                                           cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                               >  2:493335/1‑95 (MQ=255)
                                                                           cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                               <  1:493335/95‑1 (MQ=255)
                                                                           cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                             >  1:1217689/1‑129 (MQ=255)
                                                                           cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                                             <  2:1217689/129‑1 (MQ=255)
                                                                           cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                   >  2:255332/1‑139 (MQ=255)
                                                                           cGGTTGAATGAACGTCCTGTTCCCGGTGGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgaga                                                   >  1:870237/1‑139 (MQ=255)
                                                                                    gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGTCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  1:846994/139‑1 (MQ=255)
                                                                                    gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  2:902545/139‑1 (MQ=255)
                                                                                    gaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  1:255332/139‑1 (MQ=255)
                                                                                      aCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGccccca                                                                                                                                <  1:295288/52‑2 (MQ=255)
                                                                                      aCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGccccca                                                                                                                                >  2:295288/1‑51 (MQ=255)
                                                                                       cGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGccc                                                                                            >  1:270234/1‑86 (MQ=255)
                                                                                       cGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGccc                                                                                            <  2:270234/86‑1 (MQ=255)
                                                                                          ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                  <  1:657981/77‑1 (MQ=255)
                                                                                          ccTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                  >  2:657981/1‑77 (MQ=255)
                                                                                           cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          >  2:222191/1‑132 (MQ=255)
                                                                                           cTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGt                                          <  1:222191/132‑1 (MQ=255)
                                                                                               tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCa                                                                                                                >  1:1093512/1‑58 (MQ=255)
                                                                                               tCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCa                                                                                                                <  2:1093512/58‑1 (MQ=255)
                                                                                                        cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc                                       >  1:194907/1‑122 (MQ=255)
                                                                                                        cTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc                                       <  2:194907/122‑1 (MQ=255)
                                                                                                                   tCCTGCCCGGTTAAAAGCCCCCACCGCAGCGGAGGGCAAATGGGAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGccc           >  1:899909/1‑139 (MQ=255)
                                                                                                                            ggtAAAAGCCCCCACCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCa  >  2:65353/3‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCA  >  NC_000913/3308869‑3309132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: