| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,002,224 | (G)7→8 | coding (418/1071 nt) | elfG → | putative fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,002,217 | 1 | . | G | 93.5% | 100.6 / 0.4 | 31 | coding (411/1071 nt) | elfG | putative fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (16/13); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01 | |||||||||||
TGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTC > NC_000913/1002080‑1002340 | tGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑g > 2:1306768/1‑139 (MQ=255) gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGa < 1:1003709/117‑1 (MQ=255) gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGa > 2:1003709/1‑117 (MQ=255) gACAACTGGTACAAAATTAATGACTATCTTGCCGCAAAAACTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGCCACAGGGGGGGGATACCCGATGGCATATTTGCGACCATGGCGGt > 2:1113717/1‑139 (MQ=255) aaCTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCaa > 1:495566/1‑139 (MQ=255) gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCa < 1:590309/112‑1 (MQ=255) gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCa > 2:590309/1‑112 (MQ=255) aCAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAg < 2:615262/139‑1 (MQ=255) aaaaTTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGt > 1:499752/1‑139 (MQ=255) aTGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAggg > 2:1057604/1‑139 (MQ=255) cTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCg < 1:1057604/139‑1 (MQ=255) ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGa < 1:715454/81‑1 (MQ=255) ccAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGa > 2:715454/1‑81 (MQ=255) aaaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAAt > 2:683682/1‑136 (MQ=255) aaaGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAAt < 1:683682/136‑1 (MQ=255) ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGt > 2:653579/1‑101 (MQ=255) ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGt < 1:653579/101‑1 (MQ=255) ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATAcc > 2:960408/1‑50 (MQ=255) ttCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATAcc < 1:960408/50‑1 (MQ=255) gCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGa > 2:193440/1‑43 (MQ=255) gCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGa < 1:193440/43‑1 (MQ=255) gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTccc > 1:930338/1‑113 (MQ=255) gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTccc < 2:930338/113‑1 (MQ=255) gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTTGTTGGc > 1:581663/1‑139 (MQ=255) gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc > 2:130612/1‑139 (MQ=255) gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGc > 2:280524/1‑139 (MQ=255) ccTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCg < 2:499752/139‑1 (MQ=255) gAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcgtc < 2:1091947/139‑1 (MQ=255) gggCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCttt < 1:683816/92‑1 (MQ=255) gggCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCttt > 2:683816/1‑92 (MQ=255) ggCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAg > 1:697042/1‑56 (MQ=255) ggCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAg < 2:697042/56‑1 (MQ=255) | TGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTC > NC_000913/1002080‑1002340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |