Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,754	(C)5→6	coding (915/1041 nt)	galS ←	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,749	1	.	C	100.0%	164.1 / NA	46	A307G (GCA→GGA)	galS	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/24);  total (22/24)

ATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240617‑2240880                                                                                                                                      |                                                                                                                                    aTTGGAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCCCATTCTGGCGTGTTGCGACAGAATGGCGCTGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccc                                                                                                                               >  2:11770/1‑138 (MQ=255) aTTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccc                                                                                                                               >  2:1005286/1‑138 (MQ=255)    gAAAGCGGTTACATCGCCTGATGTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccctgc                                                                                                                            <  1:501644/139‑5 (MQ=255)     aaaGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccctgca                                                                                                                           >  1:1024653/1‑134 (MQ=255)                aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCg                                                                                                                 <  2:1024653/139‑1 (MQ=255)                    ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTgg                                                                                                             >  1:1039927/1‑139 (MQ=255)                    ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc                                                                                                                                  >  1:1319642/1‑117 (MQ=255)                    ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc                                                                                                                                  <  2:1319642/117‑1 (MQ=255)                      tGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCt                                                                                                           >  1:789405/1‑139 (MQ=255)                            gttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTc                                                                                                     <  2:633796/139‑1 (MQ=255)                              tgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGc                                                                                                   >  2:293563/1‑139 (MQ=255)                                 gTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAt                                                                                                <  2:459393/139‑1 (MQ=255)                                  ttAGTGATCGCCGCCGCATTCTGGCCTGTTGCGACAGAATGGCGACCCACTAACGTCCGCATAAAACAGTGGCTGGCCCGAGGATCAATAAAGCCAGCTGCCCCCCTGCCAGGCCAGTCCGGTGGCTAATTCCCCCAtt                                                                                               >  2:1336845/1‑139 (MQ=255)                                    aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             <  1:1016425/139‑1 (MQ=255)                                    aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             <  2:789405/139‑1 (MQ=255)                                    aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             <  1:152429/139‑1 (MQ=255)                                           gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCGATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATg                                                                                      >  2:852100/1‑139 (MQ=255)                                                cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGtt                                                                                                                   >  2:627232/1‑105 (MQ=255)                                                cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGtt                                                                                                                   <  1:627232/105‑1 (MQ=255)                                                     tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:440055/139‑1 (MQ=255)                                                     tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:1005286/139‑1 (MQ=255)                                                       tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:557488/137‑1 (MQ=255)                                                       tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            >  2:557488/1‑137 (MQ=255)                                                             gtTGCGACAGAATGGCGATGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGtt                                                                    <  1:11770/139‑1 (MQ=255)                                                               tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTaa                                                                  >  2:491029/1‑139 (MQ=255)                                                                   aCAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGc                                                                                                                        <  1:276379/81‑1 (MQ=255)                                                                   aCAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGc                                                                                                                        >  2:276379/1‑81 (MQ=255)                                                                      gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg                                                           <  1:293563/139‑1 (MQ=255)                                                                       aaTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGt                                                          >  1:1157385/1‑139 (MQ=255)                                                                         tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTg                                                               <  2:723387/132‑1 (MQ=255)                                                                         tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTg                                                               >  1:723387/1‑132 (MQ=255)                                                                         tGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGACACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg                                                        <  1:375106/139‑1 (MQ=255)                                                                               cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccctgc                                                                                                                            >  1:598208/1‑60 (MQ=255)                                                                               cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑cccccctgc                                                                                                                            <  2:598208/64‑5 (MQ=255)                                                                                         tCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATgg                                        <  2:1293295/139‑1 (MQ=255)                                                                                          cGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            >  2:84246/1‑102 (MQ=255)                                                                                          cGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:84246/102‑1 (MQ=255)                                                                                             cATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAat                                    >  1:1159257/1‑139 (MQ=255)                                                                                                         gctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                  <  2:311542/113‑1 (MQ=255)                                                                                                         gctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa                                                  >  1:311542/1‑113 (MQ=255)                                                                                                          ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCTATGGGAATATCATCGAAACCg                       >  1:791098/1‑139 (MQ=255)                                                                                                            ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCgg                                                                                                                >  2:1115074/1‑48 (MQ=255)                                                                                                            ggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCgg                                                                                                                <  1:1115074/48‑1 (MQ=255)                                                                                                              cACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatga                   <  2:30556/139‑1 (MQ=255)                                                                                                              cACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatga                   <  2:1159257/139‑1 (MQ=255)                                                                                                                             tGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgcg              >  1:872902/1‑127 (MQ=255)                                                                                                                             tGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag              <  2:872902/129‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAAc                                                 <  2:979493/92‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAAc                                                 >  1:979493/1‑92 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      <  2:540152/119‑1 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      >  1:540152/1‑119 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  2:482415/1‑139 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  2:1284839/1‑139 (MQ=255)                                                                                                                               ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  1:707752/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    ATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240617‑2240880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑