Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,767,438 | 1 | . | C | 95.2% | 136.3 / ‑0.2 | 42 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (19/21); total (20/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCG > NC_000913/2767305‑2767563 | tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCCAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AA‑ccccc < 1:442069/139‑1 (MQ=255) gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTcaca < 1:419322/139‑1 (MQ=255) ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc < 1:726143/139‑1 (MQ=255) cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTcc < 2:687740/139‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCGGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTcaca < 2:916503/120‑1 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTcaca > 1:916503/1‑120 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc > 2:949315/1‑117 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc < 1:1270211/117‑1 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc > 2:1270211/1‑117 (MQ=255) gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc < 1:949315/117‑1 (MQ=255) tGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTcaca < 1:1095714/107‑1 (MQ=255) tGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTcaca > 2:1095714/1‑107 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 1:1193997/114‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 2:1193997/1‑114 (MQ=255) gtataagagacaggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc < 1:1009376/126‑1 (MQ=255) taagagacagctataGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCACTC‑CC‑CCCCCCCTCACAACGTCCTGCCTAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTCcccccc < 2:144029/128‑1 (MQ=255) aaCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc < 1:449674/83‑1 (MQ=255) aaCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc > 2:449674/1‑83 (MQ=255) aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AA‑ccccccc < 2:895994/61‑1 (MQ=255) aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AA‑ccccccc > 1:895994/1‑61 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TTAAA‑CCCCAACTCGCAACGTCCTGAAAAGGGAATACGATCTTCACTGTGGGACCACAACTAAATTTCTCCCCCTTTGAACtttctggccctta > 1:144029/1‑128 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCTTTCATAGGGAACACGATTTCCCCTGTGCACCAACGACTAAATTTCTCCCCCTTTAAACTACTCaataattt > 1:391833/1‑137 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:316999/90‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:316999/1‑90 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:1278071/90‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:1278071/1‑90 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCTCATGTGAATGCACGACTAAATTTCCCCCTCACTAAAcctgtctgttatac > 2:1009376/1‑126 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt > 2:500582/1‑106 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACt < 1:500582/106‑1 (MQ=255) gTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg > 2:241281/1‑139 (MQ=255) gTCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTACCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg > 2:911981/1‑139 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTcaca > 1:809231/1‑37 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc > 2:741148/1‑42 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTc < 1:741148/42‑1 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 2:120742/1‑65 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:1000327/65‑1 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:411589/65‑1 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:411589/1‑65 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc < 1:120742/65‑1 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:1000327/1‑65 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCt < 1:487840/95‑1 (MQ=255) tCCAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACAAGTTTTCCCCTCTGCACCCACGACTAAATTTCCCCCCCt > 2:487840/1‑95 (MQ=255) cAATATGATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg < 1:454525/139‑1 (MQ=255) gATCAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 1:1343333/139‑1 (MQ=255) cAAATAGCA‑TT‑AACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg < 1:911981/139‑1 (MQ=255) | TCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCA‑TT‑AA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCG > NC_000913/2767305‑2767563 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |