| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,101,039 | (C)7→8 | coding (585/720 nt) | yggN ← | DUF2884 family putative periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,101,032 | 1 | . | C | 97.0% | 105.4 / ‑6.3 | 34 | L198V (CTG→GTG) | yggN | DUF2884 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (16/16); minor base T (1/0); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100900‑3101165 | agGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCC‑‑AGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccc‑cccc > 1:756837/1‑138 (MQ=255) ggATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccc‑ccc > 2:1281208/1‑139 (MQ=255) gATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccc‑cccc > 1:983086/1‑138 (MQ=255) tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑cccc‑ccccagg < 1:1281208/139‑5 (MQ=255) aaTTCCCGACCAGGGCTTTGCGGCTATCTTCC‑‑AGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGc < 2:756837/139‑1 (MQ=255) ttCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAc < 1:65695/132‑1 (MQ=255) ttCCAGAGTCACAACGCGGCTACAAACATCCTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGGTTGGATTGAGGATTGCAGTCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAc > 2:65695/1‑132 (MQ=255) tCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCa > 1:276772/1‑139 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGc < 2:381773/103‑1 (MQ=255) acaACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGc > 1:381773/1‑103 (MQ=255) aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGcc < 1:846356/139‑1 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:983086/139‑1 (MQ=255) ccAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 1:711671/139‑1 (MQ=255) ctgGGAATCTTTTTCCTGGTTTTTCCACTCGGTTTGGATTTAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACAtt < 1:109419/76‑1 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACAtt > 2:109419/1‑76 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc > 1:1291668/1‑136 (MQ=255) ctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc < 2:1291668/136‑1 (MQ=255) cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc > 1:188056/1‑139 (MQ=255) tttCCACTCGGTTTGGATTGAGGATTGCAG‑cccc‑ccccagg > 1:681446/1‑37 (MQ=255) tttCCACTCGGTTTGGATTGAGGATTGCAG‑cccc‑ccccagg < 2:681446/41‑5 (MQ=255) ttCCACTCGGTTTGGATTGAGGATTGCAG‑CCCCGCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGa < 2:276772/139‑1 (MQ=255) tCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg > 1:643646/1‑103 (MQ=255) tCGGTTTGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg < 2:643646/103‑1 (MQ=255) tttGGATTGAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAAtt > 1:214139/1‑139 (MQ=255) gAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACAtt < 1:636375/36‑1 (MQ=255) gAGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACAtt > 2:636375/1‑36 (MQ=255) aGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg > 1:1112915/1‑54 (MQ=255) aGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgccg < 2:1112915/54‑1 (MQ=255) aGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgc < 2:1221997/52‑1 (MQ=255) aGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccgc > 1:1221997/1‑52 (MQ=255) aGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt > 2:537356/1‑139 (MQ=255) aGGATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCtt < 1:537356/139‑1 (MQ=255) ggATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCAcc < 2:782700/109‑1 (MQ=255) ggATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCAcc > 1:782700/1‑109 (MQ=255) ggATTGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTc > 2:1108211/1‑139 (MQ=255) tGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt < 2:452782/75‑1 (MQ=255) tGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCAt > 1:452782/1‑75 (MQ=255) tGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg < 2:188056/139‑1 (MQ=255) tGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg > 2:1324885/1‑139 (MQ=255) tGCAGCCCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg > 2:924435/1‑139 (MQ=255) | AGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑CCCC‑CCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCG > NC_000913/3100900‑3101165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |