| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,728,118 | (C)8→9 | coding (1254/1455 nt) | xylB ← | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,728,110 | 1 | . | C | 91.7% | 71.6 / 0.6 | 24 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (10/12); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑C‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATG > NC_000913/3727976‑3728231 | gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑ccc < 1:323336/139‑1 (MQ=255) aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑cccc < 1:748660/139‑1 (MQ=255) ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCACCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑ccccccccgt > 2:1260009/1‑136 (MQ=255) gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCACCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑ccccccccgta < 1:1260009/139‑5 (MQ=255) tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑ccccccccgcacgg > 2:708437/1‑132 (MQ=255) aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat > 1:1097879/1‑139 (MQ=255) aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGACCCCACAT‑CTCCCCCCCGTACGGTAATCGAGCTGACGAcccgtggctt > 2:316161/1‑131 (MQ=255) aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAgct > 1:1137712/1‑115 (MQ=255) aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAgct < 2:1137712/115‑1 (MQ=255) gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt < 2:1097879/139‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑c < 1:509219/87‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑c > 2:509219/1‑87 (MQ=255) gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc > 2:69788/1‑139 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑cccc > 2:274944/1‑88 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c‑cccc < 1:274944/88‑1 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa > 2:1209834/1‑139 (MQ=255) gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAACCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc > 1:774506/1‑139 (MQ=255) gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAACCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc < 2:774506/139‑1 (MQ=255) cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCCGATATCCGCCAGCATCTGACCCCAGTACTCACTACGcgcccc > 1:292005/1‑139 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 1:864973/139‑1 (MQ=255) tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc > 1:790031/1‑123 (MQ=255) tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc < 2:790031/123‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 2:487663/100‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 1:487663/1‑100 (MQ=255) aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt < 1:316161/139‑1 (MQ=255) cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 2:292005/139‑1 (MQ=255) cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:1209834/139‑1 (MQ=255) cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑C‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa > 2:1150072/1‑139 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg > 2:510240/1‑130 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:510240/130‑1 (MQ=255) cAGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg < 1:69788/139‑1 (MQ=255) aGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg > 2:1238861/1‑63 (MQ=255) aGTGCTGGCCCCACATCC‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg < 1:1238861/63‑1 (MQ=255) | GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑C‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATG > NC_000913/3727976‑3728231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |