Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,614,614	Δ3 bp	coding (681‑683/849 nt)	focB →	putative formate transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2614614	2614616	3	56 [0]	[0] 57	focB	putative formate transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2614613	0 (0.000)	56 (1.050)	43/202	0.1	100%	coding (680/849 nt)	focB	putative formate transporter
	?	NC_000913	2614617 =	0 (0.000)					coding (684/849 nt)	focB	putative formate transporter

TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2614476‑2614613 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  >  NC_000913/2614617‑2614752                                                                                                                                                                                                                                                                                    TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCC                                                                                                                                         >  1:125382/1‑139     TGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCT                                                                                                                                     >  2:863799/1‑139     TGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCT                                                                                                                                     >  2:1167706/1‑139              TCGTTATGCGACAAAATCGTCGCCATGATTTTTCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                            <  2:101548/139‑1              TCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGC                                                                                                                            <  2:125382/139‑1                    TGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGC                                                                                                                      >  2:281145/1‑139                        ACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCC                                                                                                                                       <  1:549820/118‑1                        ACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCC                                                                                                                                       >  2:549820/1‑118                            AATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGC                                                                                                              >  1:613404/1‑139                              TCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGC                                                                                                            >  2:443856/1‑139                                GTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAG                                                                                                          <  1:863799/139‑1                                        GATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGC                                                                                                            <  2:1265524/129‑1                                        GATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGC                                                                                                            >  1:1265524/1‑129                                                  ATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGA                                                                                        <  1:443856/139‑1                                                          GTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGC                                                                                <  2:646253/139‑1                                                           TTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCA                                                                                                           <  2:1013735/111‑1                                                           TTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCA                                                                                                           >  1:1013735/1‑111                                                            TTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCA                                                                              <  1:809586/139‑1                                                              GTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATT                                                                            >  2:1044975/1‑139                                                              GTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATT                                                                            >  2:1116222/1‑139                                                              GTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATT                                                                            >  2:1319100/1‑139                                                                CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTT                                                                          >  1:498197/1‑139                                                                CGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTT                                                                          >  1:321727/1‑139                                                                  CCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTAT                                                                        <  1:1044975/139‑1                                                                  CCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTAT                                                                        <  1:1116222/139‑1                                                                    AGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTA                                                                      <  1:281145/139‑1                                                                       GGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCG                                                                   <  2:321727/139‑1                                                                                  CTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTTGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTC                                                        >  1:600993/1‑139                                                                                   TGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCC                                                       >  2:180199/1‑139                                                                                     TATCTCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCG                                                     <  2:384465/139‑1                                                                                      ATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGG                                                    <  2:613404/139‑1                                                                                                         TTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTAT                                 <  1:180199/139‑1                                                                                                         TTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAAT                                                              <  2:873794/110‑1                                                                                                         TTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAAT                                                              >  1:873794/1‑110                                                                                                             GTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGG                                                                                     >  1:670604/1‑83                                                                                                             GTTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGG                                                                                     <  2:670604/83‑1                                                                                                              TTCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCG                            >  2:691233/1‑139                                                                                                               TCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACGGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTAT                                                                        <  2:1268997/94‑1                                                                                                               TCGCCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACGGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTAT                                                                        >  1:1268997/1‑94                                                                                                                  CCATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGC‑GGGTAATATTATCGGCGGTGCG                       <  1:1319100/139‑1                                                                                                                   CATTGCCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCG                       <  1:1145404/139‑1                                                                                                                      TGCCATTCGCCATTTTGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTG                    <  2:600993/139‑1                                                                                                                        CCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTG                                                           <  1:210588/98‑1                                                                                                                        CCATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTG                                                           >  2:210588/1‑98                                                                                                                         CATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTG                 <  2:498197/139‑1                                                                                                                         CATTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTG                 <  1:1167706/139‑1                                                                                                                           TTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGT               >  2:453901/1‑139                                                                                                                           TTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGT               >  2:1165835/1‑139                                                                                                                           TTCGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGAAATATTATCGGCGGTGCGGTGCTGGT               >  1:1083459/1‑139                                                                                                                             CGCCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGA             >  1:662543/1‑139                                                                                                                               CCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTG                    <  2:667308/130‑1                                                                                                                               CCATTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTG                    >  1:667308/1‑130                                                                                                                                  TTTCGCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATG        >  1:1134239/1‑139                                                                                                                                       CCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTA   <  2:1134239/139‑1                                                                                                                                       CCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTA   <  1:453901/139‑1                                                                                                                                        CCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  <  1:691233/139‑1                                                                                                                                                                                                                                                                                    TTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTGTTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2614476‑2614613 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTAT  >  NC_000913/2614617‑2614752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.