Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	616,422	A→G	S756G (AGC→GGC)	entF →	enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	616,422	0	A	G	100.0%	233.5 / NA	65	S756G (AGC→GGC)	entF	enterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

AACAGGTTTTCTGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616306‑616556                                                                                                                     |                                                                                                                                       aaCAGGTTTTCTGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGa                                                                                                                  <  1:169035/139‑1 (MQ=255)                   gtgAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                >  2:129841/1‑139 (MQ=255)                   gtgAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                >  1:55522/1‑139 (MQ=255)                        gCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgca                                                                                           >  2:619560/1‑139 (MQ=255)                           ttACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        <  1:619560/139‑1 (MQ=255)                           ttACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        >  1:130922/1‑139 (MQ=255)                             aCCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGcagcagg                                                                                      >  1:682798/1‑138 (MQ=255)                                     aTTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGAt                                                                              <  2:130922/139‑1 (MQ=255)                                           gCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGc                                                                                                                                      >  1:1088095/1‑77 (MQ=255)                                               cggaTGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTgg                                                                                                          <  1:899798/98‑1 (MQ=255)                                               cgAATGGCAACAGTTTACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTGGGCGAGGAACTgg                                                                                                          >  2:899798/1‑101 (MQ=255)                                               cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCg                                                                                                                   <  1:1150994/92‑1 (MQ=255)                                               cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCg                                                                                                                   >  2:1150994/1‑92 (MQ=255)                                               cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTgg                                                                                                          <  1:76925/101‑1 (MQ=255)                                               cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTgg                                                                                                          >  2:76925/1‑101 (MQ=255)                                               cgAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCg                                                                    >  1:1005311/1‑139 (MQ=255)                                                  aTGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 <  1:129841/139‑1 (MQ=255)                                                              aaCTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 <  2:163810/127‑1 (MQ=255)                                                              aaCTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 >  1:163810/1‑127 (MQ=255)                                                                    cgcgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagc                                                                                         >  2:1252794/1‑97 (MQ=255)                                                                    cgcgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagc                                                                                         <  1:1252794/97‑1 (MQ=255)                                                                    cgcgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGt                                               >  2:426872/1‑139 (MQ=255)                                                                      cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTg                                                                                                                                    >  2:163116/1‑52 (MQ=255)                                                                      cgcCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTg                                                                                                                                    <  1:163116/52‑1 (MQ=255)                                                                             gCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGAt                                      <  2:55522/139‑1 (MQ=255)                                                                                      atatGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGatgatg                             >  1:441014/1‑139 (MQ=255)                                                                                        atGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCAGAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCa                           >  2:881280/1‑139 (MQ=255)                                                                                        atGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCa                           >  2:684639/1‑139 (MQ=255)                                                                                        atGGCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCa                           >  2:22982/1‑139 (MQ=255)                                                                                          ggCCCGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATc                         <  2:1005311/139‑1 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                >  1:316298/1‑66 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTgcg                                                                                                <  2:316298/66‑1 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        <  1:868751/74‑1 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        >  2:868751/1‑74 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcag                                                                                          >  2:732711/1‑72 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcag                                                                                          <  1:732711/72‑1 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTAt                                                                       <  2:1182483/91‑1 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTAt                                                                       >  1:1182483/1‑91 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 >  2:736080/1‑97 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTa                                                                 <  1:736080/97‑1 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACggg                                                      >  2:1235063/1‑108 (MQ=255)                                                                                            cccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACggg                                                      <  1:1235063/108‑1 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAAc                                                                                                             >  2:934455/1‑52 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAAc                                                                                                             <  1:934455/52‑1 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTgg                                                                           >  2:407191/1‑86 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTgg                                                                           <  1:407191/86‑1 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACgggg                                                     >  2:786397/1‑107 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGt                                                     <  1:786397/108‑1 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:658291/1‑139 (MQ=255)                                                                                             ccGACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCgg                      >  2:673556/1‑139 (MQ=255)                                                                                               gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        >  2:741813/1‑71 (MQ=255)                                                                                               gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGgcagca                                                                                        <  1:741813/71‑1 (MQ=255)                                                                                               gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATg                                                               <  1:1161087/96‑1 (MQ=255)                                                                                               gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATg                                                               >  2:1161087/1‑96 (MQ=255)                                                                                               gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGa                                       <  1:751228/120‑1 (MQ=255)                                                                                               gACGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGa                                       >  2:751228/1‑120 (MQ=255)                                                                                                 cGGAAGCGGCGGTAGATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTgcc                  >  2:1082311/1‑139 (MQ=255)                                                                                                               gATGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCgg    <  1:22982/139‑1 (MQ=255)                                                                                                                aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  1:881280/139‑1 (MQ=255)                                                                                                                aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  2:441014/139‑1 (MQ=255)                                                                                                                aTGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCggg   <  1:1236358/139‑1 (MQ=255)                                                                                                                 tGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt  <  1:684639/139‑1 (MQ=255)                                                                                                                 tGTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt  <  1:426872/139‑1 (MQ=255)                                                                                                                  gTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    >  1:908383/1‑40 (MQ=255)                                                                                                                  gTCGGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAgg                                                                                                    <  2:908383/40‑1 (MQ=255)                                                                                                                     |                                                                                                                                       AACAGGTTTTCTGTAGTGGTGAGGCCTTACCGGCTGATTTATGCCGCGAATGGCAACAGTTAACTGGCGCGCCGTTGCATAATCTATATGGCCCGACGGAAGCGGCGGTAGATGTCAGCTGGTATCCGGCTTTTGGCGAGGAACTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGT  >  NC_000913/616306‑616556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑