Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 56,296 A→G W272R (TGG→CGG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091356,2960AG100.0% 235.4 / NA 66W272R (TGG→CGG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/33);  total (33/33)

GTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATC  >  NC_000913/56162‑56423
                                                                                                                                      |                                                                                                                               
gTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCatg                                                                                                                             >  2:1128473/1‑139 (MQ=255)
     aGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGtt                                                                                                                        >  2:442332/1‑139 (MQ=255)
           aTAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  <  1:1254662/139‑1 (MQ=255)
             aaCCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgac                                                                                                                <  1:442332/139‑1 (MQ=255)
                           aCTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATgc                                                                                                  >  1:513667/1‑139 (MQ=255)
                              gTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  <  2:875722/120‑1 (MQ=255)
                              gTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  >  1:875722/1‑120 (MQ=255)
                                cATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGt                                                                                             >  2:493131/1‑139 (MQ=255)
                                      tCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggt                                                                                       <  1:597520/139‑1 (MQ=255)
                                        gggTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggtgg                                                                                     <  1:1128473/139‑1 (MQ=255)
                                             ttCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATcc                                                                                >  1:268417/1‑139 (MQ=255)
                                                    tCATAGACTTTATCCGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATg                                                                                                   <  2:78950/113‑1 (MQ=255)
                                                    tCATAGACTTTATCCGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATg                                                                                                   >  1:78950/1‑113 (MQ=255)
                                                       tAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgacg                                                                                                               >  1:210110/1‑98 (MQ=255)
                                                       tAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgacg                                                                                                               <  2:210110/98‑1 (MQ=255)
                                                        aGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcgc                                                                     <  1:113265/139‑1 (MQ=255)
                                                        aGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcgc                                                                     >  2:254400/1‑139 (MQ=255)
                                                          aCTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGa                                                                   <  1:254400/139‑1 (MQ=255)
                                                            tttATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggtgg                                                                                     >  2:812052/1‑119 (MQ=255)
                                                            tttATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggtgg                                                                                     <  1:812052/119‑1 (MQ=255)
                                                               aTCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  >  1:174341/1‑87 (MQ=255)
                                                               aTCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  <  2:174341/87‑1 (MQ=255)
                                                                tCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTcc                                                             <  1:497275/139‑1 (MQ=255)
                                                                      ggCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggtgg                                                                                     >  1:859338/1‑109 (MQ=255)
                                                                      ggCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggtgg                                                                                     <  2:859338/109‑1 (MQ=255)
                                                                      ggCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAat                                                       >  1:110897/1‑139 (MQ=255)
                                                                         aGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgacg                                                                                                               <  2:133894/80‑1 (MQ=255)
                                                                         aGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgacg                                                                                                               >  1:133894/1‑80 (MQ=255)
                                                                              gTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATgc                                                                                                  <  2:429674/88‑1 (MQ=255)
                                                                              gTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATgc                                                                                                  >  1:429674/1‑88 (MQ=255)
                                                                                ccAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTa                                             <  2:110897/139‑1 (MQ=255)
                                                                                 cAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  >  2:350603/1‑69 (MQ=255)
                                                                                 cAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacg                                                                                                                  <  1:350603/69‑1 (MQ=255)
                                                                                 cAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGAt                                                                                                             >  1:846553/1‑74 (MQ=255)
                                                                                 cAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGAt                                                                                                             <  2:846553/74‑1 (MQ=255)
                                                                                    ttCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAAc                                         >  1:961440/1‑139 (MQ=255)
                                                                                     tCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACt                                        >  1:913120/1‑139 (MQ=255)
                                                                                      ccATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggt                                                                                       >  2:1289753/1‑91 (MQ=255)
                                                                                      ccATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggt                                                                                       <  1:1289753/91‑1 (MQ=255)
                                                                                       cATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCa                                      <  2:913120/139‑1 (MQ=255)
                                                                                       cATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCa                                      >  1:790934/1‑139 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCcgca                                                                                                                               <  2:340634/48‑1 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCcgca                                                                                                                               >  1:340634/1‑48 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgacg                                                                                                               >  2:1274168/1‑64 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCacgacg                                                                                                               <  1:1274168/64‑1 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGa                                                                   >  1:762018/1‑108 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGa                                                                   <  2:762018/108‑1 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTa                                                         >  2:1226197/1‑118 (MQ=255)
                                                                                         tCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTa                                                         <  1:1226197/118‑1 (MQ=255)
                                                                                          cacgCCAGTGCCTGCCTGGGAGAGCTAGTGGAGTGCGTGCTCCCGCATGATGTTGCCACGACGATGCATATAATGCCGCGTGATGGTGTCATCAATATTTGGCGCGATGTTCCAGTAATGTGGCAGGTAGAACTCAAag                                   <  2:464310/136‑1 (MQ=255)
                                                                                              ccAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAat                                                       >  1:43474/1‑115 (MQ=255)
                                                                                              ccAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAat                                                       <  2:43474/115‑1 (MQ=255)
                                                                                              ccAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAagtagt                               >  2:348053/1‑139 (MQ=255)
                                                                                               cAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGATGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCTAAGTAGtt                              >  2:817366/1‑139 (MQ=255)
                                                                                                 gcgcCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTtgg                            <  2:513667/139‑1 (MQ=255)
                                                                                                  cgcccgccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCAcgacga                                                                                                              >  1:67916/1‑56 (MQ=255)
                                                                                                  cgcccgccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCAcgacga                                                                                                              <  2:67916/56‑1 (MQ=255)
                                                                                                   gcccgccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTg                                                                                            >  2:1028479/1‑73 (MQ=255)
                                                                                                   gcccgccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTg                                                                                            <  1:1028479/73‑1 (MQ=255)
                                                                                                        ccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGAt                                                                                                             <  2:724269/51‑1 (MQ=255)
                                                                                                        ccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGAt                                                                                                             >  1:724269/1‑51 (MQ=255)
                                                                                                        ccTGGGAGAGGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTggtggtggt                     >  1:596791/1‑139 (MQ=255)
                                                                                                               gagGTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTg              <  2:1287341/139‑1 (MQ=255)
                                                                                                                 ggTAGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGc            >  1:182024/1‑139 (MQ=255)
                                                                                                                   tAGCGGAATTCGTTCTCCCGCATGATGTTGCCATGGTGGTGAATATAATACGGCGAGATGGTGGCATCCATATTTGGCGCAATGTGCCAGTAACATGGCAGGTAGAGCTCAAAGTAGTTGGTGGTGGTGTACTTGGAGt          <  2:596791/139‑1 (MQ=255)
                                                                                                                    aGCGGAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGtt         <  1:493131/139‑1 (MQ=255)
                                                                                                                        gAATTCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCggg     <  1:348053/139‑1 (MQ=255)
                                                                                                                           ttCGTTCTCCCGCATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATc  >  2:526777/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                               
GTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGAACTCAAAGTAGTTGGTGGTGGTGTACTTGGCGTTCGGGATC  >  NC_000913/56162‑56423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑