Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	664,102	C→T	*363* (TAG→TAA)	rlpA ←	septal ring protein, suppressor of prc, minor lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	664,102	0	C	T	100.0%	210.3 / NA	65	*363* (TAG→TAA)	rlpA	septal ring protein, suppressor of prc, minor lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/33);  total (32/33)

AACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCG  >  NC_000913/663970‑664216                                                                                                                                     |                                                                                                                   aaCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              >  2:339933/1‑139 (MQ=255)  aCTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             <  2:664718/139‑1 (MQ=255)              gTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              >  2:65097/1‑126 (MQ=255)              gTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              <  1:65097/126‑1 (MQ=255)                     aaTTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTg                                                                                          >  2:1009356/1‑139 (MQ=255)                      aTTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGt                                                                                         >  1:462742/1‑139 (MQ=255)                        tAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTaa                                                                                       <  1:183684/139‑1 (MQ=255)                              aGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGc                                                                                 <  1:784988/139‑1 (MQ=255)                                               aaTGCACTACCGGCTGGCATCTGACTTTCCGCTTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATCAAt                                                                                               <  2:865321/108‑1 (MQ=255)                                               aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             <  2:180825/94‑1 (MQ=255)                                               aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             >  1:180825/1‑94 (MQ=255)                                               aaTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAAt                                                                                               >  1:865321/1‑108 (MQ=255)                                                aTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              <  1:909851/92‑1 (MQ=255)                                                aTGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              >  2:909851/1‑92 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             <  1:1184217/92‑1 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             >  2:1184217/1‑92 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                           >  2:1319318/1‑94 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGcgcg                                                                                                           <  1:1319318/94‑1 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaa                                                                                                    <  1:411331/101‑1 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGtaa                                                                                                    >  2:411331/1‑101 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCtt                                                                               >  1:349119/1‑122 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCtt                                                                               <  2:349119/122‑1 (MQ=255)                                                 tGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgc                                                              >  1:484598/1‑139 (MQ=255)                                                 tGCACTACCGGCAAGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGCCACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              <  2:449344/91‑1 (MQ=255)                                                 tGCACTACCGGCAAGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              >  1:449344/1‑91 (MQ=255)                                                  gCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                         >  1:1036338/1‑95 (MQ=255)                                                  gCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                         <  2:1036338/95‑1 (MQ=255)                                                     cTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                          <  2:462742/139‑1 (MQ=255)                                                      tACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              >  1:302127/1‑86 (MQ=255)                                                      tACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgc                                                                                                              <  2:302127/86‑1 (MQ=255)                                                      tACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCaa                                                         >  1:661078/1‑139 (MQ=255)                                                       aCCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             >  2:612141/1‑86 (MQ=255)                                                       aCCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             <  1:612141/86‑1 (MQ=255)                                                             aggcaTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             <  2:180708/80‑1 (MQ=255)                                                             aggcaTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTgcg                                                                                                             >  1:180708/1‑80 (MQ=255)                                                                caTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCgg                                                                                                          >  2:448091/1‑80 (MQ=255)                                                                caTCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCgg                                                                                                          <  1:448091/80‑1 (MQ=255)                                                                  tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCa                                                                      <  1:131614/114‑1 (MQ=255)                                                                  tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCa                                                                      >  2:131614/1‑114 (MQ=255)                                                                  tCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTc                                             >  2:1114460/1‑139 (MQ=255)                                                                      aCTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                         <  2:516569/75‑1 (MQ=255)                                                                      aCTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgt                                                                                                         >  1:516569/1‑75 (MQ=255)                                                                           ccGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgtag                                                                                                       <  2:1324643/72‑1 (MQ=255)                                                                           ccGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGgtag                                                                                                       >  1:1324643/1‑72 (MQ=255)                                                                             gcgTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgctg                                                            <  2:1098231/113‑1 (MQ=255)                                                                             gcgTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtgctg                                                            >  1:1098231/1‑113 (MQ=255)                                                                                 gACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                              <  2:661078/139‑1 (MQ=255)                                                                                 gACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGaa                              <  1:1009356/139‑1 (MQ=255)                                                                                            cGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                               >  1:1140563/1‑95 (MQ=255)                                                                                            cGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                               <  2:1140563/95‑1 (MQ=255)                                                                                             gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                          <  1:1149808/99‑1 (MQ=255)                                                                                             gTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCa                                                          >  2:1149808/1‑99 (MQ=255)                                                                                              tCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                 <  1:339933/139‑1 (MQ=255)                                                                                                       gAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCgg                                           <  1:1201566/104‑1 (MQ=255)                                                                                                       gAAATTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCgg                                           >  2:1201566/1‑104 (MQ=255)                                                                                                        aaaTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                               >  2:61280/1‑83 (MQ=255)                                                                                                        aaaTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTtg                                                               <  1:61280/83‑1 (MQ=255)                                                                                                          aTTGACACATCAGATGCCTGCTTTACGTTACTgcgc                                                                                                            <  2:1318713/36‑1 (MQ=255)                                                                                                          aTTGACACATCAGATGCCTGCTTTACGTTACTgcgc                                                                                                            >  1:1318713/1‑36 (MQ=255)                                                                                                          aTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                 >  2:501206/1‑127 (MQ=255)                                                                                                          aTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTgg                 <  1:501206/127‑1 (MQ=255)                                                                                                          aTTGACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGAcc     >  1:889539/1‑139 (MQ=255)                                                                                                             gACACATCAGATGCCTGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACcgcg  <  2:889539/139‑1 (MQ=255)                                                                                                                            tGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTg                                      >  2:780365/1‑88 (MQ=255)                                                                                                                            tGCTTTACGTTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTg                                      <  1:780365/88‑1 (MQ=255)                                                                                                                                     |                                                                                                                   AACTACGACATCCGTGATGGAATTAAAAAAGTGCCCTACTATAGCAAATGCACTACCGGCAGGCATCTGACTTTCCGCGTGACTTTGTTAACGTCATTTACAGAAATTGACACATCAGATGCCTGCTTTACGCTACTGCGCGGTAGTAATAAATGACTGTAATTGGGCTTCGGTTTGCAAACGTTGCTGCAAGGTACTGGCTTCGGCTTTGCTGGCGAATGGGCCAAGCTGGATCCGCCAGACCGCG  >  NC_000913/663970‑664216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑