Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	690,550	A→G	V111A (GTG→GCG)	lnt ←	apolipoprotein N‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	690,550	0	A	G	100.0%	133.3 / NA	39	V111A (GTG→GCG)	lnt	apolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

TATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCG  >  NC_000913/690422‑690672                                                                                                                                 |                                                                                                                           tataGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  <  1:30907/139‑1 (MQ=255)   taGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:439859/139‑1 (MQ=255)         aaCTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGCAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  <  2:810576/131‑1 (MQ=255)         aaCTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  >  1:810576/1‑131 (MQ=255)                      ggAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  2:43111/120‑1 (MQ=255)                      ggAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  1:43111/1‑120 (MQ=255)                        aaGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAAt                                                                                           >  1:1034716/1‑139 (MQ=255)                               tCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACa                                                                                                    >  1:1071070/1‑123 (MQ=255)                               tCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACa                                                                                                    <  2:1071070/123‑1 (MQ=255)                                     cccAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCcagcag                                                                              <  2:909295/139‑1 (MQ=255)                                      ccAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTcc                                                                                                            <  1:421109/108‑1 (MQ=255)                                      ccAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTcc                                                                                                            >  2:421109/1‑108 (MQ=255)                                       cAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAgcagca                                                                            <  2:1244159/139‑1 (MQ=255)                                        aaCCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGcac                                                                           <  2:237463/139‑1 (MQ=255)                                        aaCCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGcac                                                                           >  2:1085206/1‑139 (MQ=255)                                             cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:642375/1‑97 (MQ=255)                                             cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:642375/97‑1 (MQ=255)                                             cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:817498/1‑97 (MQ=255)                                             cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:817498/97‑1 (MQ=255)                                             cgcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCAccacca                                                                      >  2:64918/1‑139 (MQ=255)                                                          tCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAAc                                                         <  2:621132/139‑1 (MQ=255)                                                                gCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:1301464/1‑78 (MQ=255)                                                                gCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:1301464/78‑1 (MQ=255)                                                                  cAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                >  2:252818/1‑76 (MQ=255)                                                                  cAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACa                                                                                                                <  1:252818/76‑1 (MQ=255)                                                                        gCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAgcagca                                                                            >  1:1305918/1‑106 (MQ=255)                                                                        gCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAgcagca                                                                            <  2:1305918/106‑1 (MQ=255)                                                                                 gcAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAgg                                  >  2:798559/1‑139 (MQ=255)                                                                                 gcAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAgg                                  >  1:2295/1‑139 (MQ=255)                                                                                   aaTCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTc                                >  2:338983/1‑139 (MQ=255)                                                                                     tCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTcgc                              <  2:2295/139‑1 (MQ=255)                                                                                       gCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGa                            <  1:1085206/139‑1 (MQ=255)                                                                                            gcgcAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGt                                                                                                              <  2:1265673/52‑1 (MQ=255)                                                                                            gcgcAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGt                                                                                                              >  1:1265673/1‑52 (MQ=255)                                                                                                aGCCAGGTGGTTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACAt                   >  2:456939/1‑139 (MQ=255)                                                                                                     ggtggtTTTCGGCCACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGAcc              <  2:1034716/139‑1 (MQ=255)                                                                                                      gtggtTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  <  2:680604/38‑1 (MQ=255)                                                                                                      gtggtTTTCGGCCACAGACGCGACAGCGCGCCAGCaaa                                                                                                                  >  1:680604/1‑38 (MQ=255)                                                                                                                 ccACAGACGCGACAGCGCGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCg  <  1:338983/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                           TATAGCCGAACTGTAACCACGGGAAGCCGGTCAGTACCCAACCGCGCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCG  >  NC_000913/690422‑690672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑