Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 816,531 G→A V39V (GTC→GTT)  ybhK ← putative CofD superfamily transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913816,5310GA100.0% 127.2 / NA 40V39V (GTC→GTT) ybhKputative CofD superfamily transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)

TATTCAAACATCGCGGAGGCGACGCTCGGTTCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTGACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTGGAGTCAG  >  NC_000913/816400‑816665
                                                                                                                                   |                                                                                                                                      
tATTCAAACATCGCGGAGGCGACGCTCGGTTCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATAc                                                                                                                                 <  1:317977/139‑1 (MQ=255)
          tCGCGGAGGCGACGCTCGGTTCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACg                                                                                                                       >  2:1227994/1‑139 (MQ=255)
          tCGCGGAGGCGACGCTCGGTTCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACg                                                                                                                       >  1:759391/1‑139 (MQ=255)
                  gCGACGCTCGGTTCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTAAAACGAGAACCCa                                                                                                               <  2:759391/139‑1 (MQ=255)
                             ttCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGt                                                                                                    <  2:595926/139‑1 (MQ=255)
                                      cAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATgagaga                                                                                           >  2:675870/1‑139 (MQ=255)
                                                 ggCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACga                                                                                                                      <  1:832738/101‑1 (MQ=255)
                                                 ggCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACga                                                                                                                      >  2:832738/1‑101 (MQ=255)
                                                      ttGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGa                                                                                                           <  2:519443/107‑1 (MQ=255)
                                                      ttGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGa                                                                                                           >  1:519443/1‑107 (MQ=255)
                                                                   cccAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAAc                                                                                         <  2:1093215/112‑1 (MQ=255)
                                                                   cccAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAAc                                                                                         >  1:1093215/1‑112 (MQ=255)
                                                                   cccAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCgag                                                              >  2:1290867/1‑139 (MQ=255)
                                                                       ggCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCa                                                          >  2:72302/1‑139 (MQ=255)
                                                                       ggCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCa                                                          >  2:76008/1‑139 (MQ=255)
                                                                        gCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCaa                                                         >  2:998029/1‑139 (MQ=255)
                                                                          aaTGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACga                                                                                                                      <  1:18962/76‑1 (MQ=255)
                                                                          aaTGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACga                                                                                                                      >  2:18962/1‑76 (MQ=255)
                                                                             gccgccTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGaca                                                    <  1:72302/139‑1 (MQ=255)
                                                                                 ccTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGaca                                                    <  1:173872/135‑1 (MQ=255)
                                                                                 ccTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGaca                                                    >  2:173872/1‑135 (MQ=255)
                                                                                 ccTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGAt                                                <  1:998029/139‑1 (MQ=255)
                                                                                 ccTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGAt                                                <  1:1290867/139‑1 (MQ=255)
                                                                                             cGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagcca                                    >  1:1089578/1‑139 (MQ=255)
                                                                                             cGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagcca                                    <  1:1227994/139‑1 (MQ=255)
                                                                                                     ccccGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGac                                                     <  1:1093057/114‑1 (MQ=255)
                                                                                                     ccccGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGac                                                     >  2:1093057/1‑114 (MQ=255)
                                                                                                      cccGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGAt                           >  1:1089387/1‑139 (MQ=255)
                                                                                                       ccGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGac                                                     >  1:115187/1‑112 (MQ=255)
                                                                                                       ccGTCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGac                                                     <  2:115187/112‑1 (MQ=255)
                                                                                                          tCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTccc                                                                                  <  2:652921/80‑1 (MQ=255)
                                                                                                          tCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTccc                                                                                  >  1:652921/1‑80 (MQ=255)
                                                                                                          tCGAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTgcg                       >  2:1159476/1‑139 (MQ=255)
                                                                                                            gAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagc                                      >  2:516965/1‑122 (MQ=255)
                                                                                                            gAGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagc                                      <  1:516965/122‑1 (MQ=255)
                                                                                                             aGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTccgcc                                                                 <  2:485829/94‑1 (MQ=255)
                                                                                                             aGCCACCATTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTccgcc                                                                 >  1:485829/1‑94 (MQ=255)
                                                                                                               ccaccaTTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagc                                      <  2:1051534/119‑1 (MQ=255)
                                                                                                               ccaccaTTATCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagc                                      >  1:1051534/1‑119 (MQ=255)
                                                                                                                        tCGGTGGTGGTAACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTg         <  2:1089387/139‑1 (MQ=255)
                                                                                                                            tggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGAt                                                <  2:441055/96‑1 (MQ=255)
                                                                                                                            tggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGAt                                                >  1:441055/1‑96 (MQ=255)
                                                                                                                            tggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagc                                      <  1:784469/106‑1 (MQ=255)
                                                                                                                            tggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATcagc                                      >  2:784469/1‑106 (MQ=255)
                                                                                                                            tggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTGGAg      >  2:1293762/1‑138 (MQ=255)
                                                                                                                            tggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTGGAg      <  1:1293762/138‑1 (MQ=255)
                                                                                                                             ggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGAt                           >  2:195919/1‑116 (MQ=255)
                                                                                                                             ggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGAt                           <  1:195919/116‑1 (MQ=255)
                                                                                                                             ggtggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTGGAGTc    >  2:1316517/1‑139 (MQ=255)
                                                                                                                               tggtaACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTGGAGTCAg  >  1:636664/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TATTCAAACATCGCGGAGGCGACGCTCGGTTCCGTTATCAGCTGGTTGAGGCAGTTGCGCATATCGCCCCAGGCAATGCCGCCTTCTGAACGGCGAATACGCCCCGTCGAGCCACCATTATCGGTGGTGGTGACGATACCCGTTAAACGAGAACCCAAAGACGAAAGTGATGAGAGAACGCGTCCCAGTCCATGCCCTCCGCCGAGAGCAACGACACGATCAAGATCAGCCAGCGTACGATTGCGCATATAGTTTCCTGGAGTCAG  >  NC_000913/816400‑816665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑