Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,115	G→A	*255* (TAG→TAA)	yabI →	DedA family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,115	0	G	A	100.0%	152.1 / NA	48	*255* (TAG→TAA)	yabI	DedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

GCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAA  >  NC_000913/71991‑72246                                                                                                                             |                                                                                                                                    gcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTtgtg                                                                                                                       >  2:85027/1‑139 (MQ=255)           gttgtGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCt                                                                                                             <  1:85027/139‑1 (MQ=255)                                                    cgcTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                                    >  1:1156965/1‑139 (MQ=255)                                                      cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCggg                                                                                                                   >  1:1194104/1‑90 (MQ=255)                                                      cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCggg                                                                                                                   >  1:255208/1‑90 (MQ=255)                                                      cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCggg                                                                                                                   <  2:255208/90‑1 (MQ=255)                                                      cTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCggg                                                                                                                   <  2:1194104/90‑1 (MQ=255)                                                         gtggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTAcc                                                               >  2:936128/1‑139 (MQ=255)                                                          tggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                     >  2:438574/1‑116 (MQ=255)                                                          tggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                     <  1:438574/116‑1 (MQ=255)                                                          tggtgTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTAccc                                                              <  2:557235/139‑1 (MQ=255)                                                                tAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGt                                                        <  2:1156965/139‑1 (MQ=255)                                                                      gCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATa                                                  >  1:986198/1‑139 (MQ=255)                                                                       ccACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATaa                                                 >  1:599712/1‑139 (MQ=255)                                                                            cGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                                    <  2:862923/115‑1 (MQ=255)                                                                            cGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCa                                                                    >  1:862923/1‑115 (MQ=255)                                                                              ttGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgcg                                                                                           >  2:991808/1‑90 (MQ=255)                                                                              ttGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgcg                                                                                           <  1:991808/90‑1 (MQ=255)                                                                              ttGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTc                                          <  2:599712/139‑1 (MQ=255)                                                                                     cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCAcgccgc                                   >  1:241270/1‑139 (MQ=255)                                                                                      ggTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                               >  2:250104/1‑78 (MQ=255)                                                                                      ggTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                               <  1:250104/78‑1 (MQ=255)                                                                                      ggTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCa                                  >  2:1261924/1‑139 (MQ=255)                                                                                          tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCg                              <  1:1261924/139‑1 (MQ=255)                                                                                           atatCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                             <  2:986198/139‑1 (MQ=255)                                                                                            tatCGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                            <  2:241270/139‑1 (MQ=255)                                                                                               cGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgcg                                                                                           >  1:921146/1‑73 (MQ=255)                                                                                               cGATATTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCcgcg                                                                                           <  2:921146/73‑1 (MQ=255)                                                                                                 atatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGt                                                            >  1:894315/1‑102 (MQ=255)                                                                                                 atatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGt                                                            <  2:894315/102‑1 (MQ=255)                                                                                                 atatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGc                       <  1:936128/139‑1 (MQ=255)                                                                                                  tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGt                                                                                                                             <  1:502417/36‑1 (MQ=255)                                                                                                  tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGt                                                                                                                             >  2:502417/1‑36 (MQ=255)                                                                                                  tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                             <  2:370730/132‑1 (MQ=255)                                                                                                  tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                             >  1:370730/1‑132 (MQ=255)                                                                                                  tatTTTGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCa                      >  1:377150/1‑139 (MQ=255)                                                                                                      ttGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATatt                  >  2:1172900/1‑137 (MQ=255)                                                                                                       tGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTa                 >  2:366394/1‑139 (MQ=255)                                                                                                       tGCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTa                 >  2:70537/1‑139 (MQ=255)                                                                                                         cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                               >  2:892246/1‑59 (MQ=255)                                                                                                         cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGc                                                                                               <  1:892246/59‑1 (MQ=255)                                                                                                         cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                     >  2:615925/1‑69 (MQ=255)                                                                                                         cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                     <  1:615925/69‑1 (MQ=255)                                                                                                            aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg            <  1:1172900/139‑1 (MQ=255)                                                                                                                      gggTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaaa                                                                         >  1:1153287/1‑68 (MQ=255)                                                                                                                      gggTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaaa                                                                         <  2:1153287/68‑1 (MQ=255)                                                                                                                        gTTTAAGAGATGGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCaa  >  2:322728/1‑137 (MQ=255)                                                                                                                        gTTTAAGAGATGGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCaa  <  1:322728/137‑1 (MQ=255)                                                                                                                             |                                                                                                                                    GCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAA  >  NC_000913/71991‑72246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑