Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 911,132 A→G intergenic (‑83/+50) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913911,1320AG100.0% 147.5 / NA 43intergenic (‑83/+50)poxB/hcrpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/23);  total (20/23)

CCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCG  >  NC_000913/911000‑911254
                                                                                                                                    |                                                                                                                          
ccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACg                                                                                                                      <  1:142047/139‑1 (MQ=255)
   gCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGtt                                                                                                                   >  2:921990/1‑139 (MQ=255)
           gAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGcc                                                                                                           <  1:511000/139‑1 (MQ=255)
             gtattttGGCGATCTAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTCCATCTGACGGAGGGGGAAGCGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg                                                                                                         <  2:888844/136‑1 (MQ=255)
                ttttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATg                                                                                                      <  2:1051122/139‑1 (MQ=255)
                  ttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                    <  1:921990/139‑1 (MQ=255)
                  tcggCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                    <  2:792254/137‑1 (MQ=255)
                                                      ctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGa                                                                                                        <  1:1045511/99‑1 (MQ=255)
                                                      ctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGa                                                                                                        >  2:1045511/1‑99 (MQ=255)
                                                              ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAg                                                                                                  >  2:820585/1‑97 (MQ=255)
                                                              ccTGAATGTGATAACGGTAACAAGGTTAGTTCATCTGACGGAGGGGGAAGGGATGGTAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAg                                                                                                  <  1:820585/97‑1 (MQ=255)
                                                                    tgtgATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa                                                                                                                        >  2:1039717/1‑69 (MQ=255)
                                                                    tgtgATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTaa                                                                                                                        <  1:1039717/69‑1 (MQ=255)
                                                                     gtgATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCg                                                 <  2:151408/139‑1 (MQ=255)
                                                                       gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATg                                                                                                      <  2:928188/84‑1 (MQ=255)
                                                                       gATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATg                                                                                                      >  1:928188/1‑84 (MQ=255)
                                                                        aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa                                                                                                                <  1:453459/73‑1 (MQ=255)
                                                                        aTAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa                                                                                                                >  2:453459/1‑73 (MQ=255)
                                                                              gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg                                                >  1:438040/1‑131 (MQ=255)
                                                                              gTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg                                                <  2:438040/131‑1 (MQ=255)
                                                                               tAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGGGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGa                                       >  2:433648/1‑139 (MQ=255)
                                                                                        tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt                                                                                                                          <  1:291633/47‑1 (MQ=255)
                                                                                        tAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCt                                                                                                                          >  2:291633/1‑47 (MQ=255)
                                                                                           ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat                                                                                         <  1:592822/77‑1 (MQ=255)
                                                                                           ttCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat                                                                                         >  2:592822/1‑77 (MQ=255)
                                                                                               tCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTaa                       >  1:14733/1‑139 (MQ=255)
                                                                                                cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                    >  2:24079/1‑61 (MQ=255)
                                                                                                cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa                                                                                                    <  1:24079/61‑1 (MQ=255)
                                                                                                cTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAAc                      >  1:225858/1‑139 (MQ=255)
                                                                                                         ggggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGa             >  1:836634/1‑139 (MQ=255)
                                                                                                          gggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                           <  2:321023/108‑1 (MQ=255)
                                                                                                          gggAAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                           >  1:321023/1‑108 (MQ=255)
                                                                                                                 gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg                                                                                                         >  2:1102379/1‑39 (MQ=255)
                                                                                                                 gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg                                                                                                         <  1:1102379/39‑1 (MQ=255)
                                                                                                                 gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc                                <  2:1334674/112‑1 (MQ=255)
                                                                                                                 gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGcc                                >  1:1334674/1‑112 (MQ=255)
                                                                                                                    gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                           <  2:171461/98‑1 (MQ=255)
                                                                                                                    gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc                                           >  1:171461/1‑98 (MQ=255)
                                                                                                                    gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCg  <  2:836634/139‑1 (MQ=255)
                                                                                                                     ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCa                                                             >  1:487411/1‑79 (MQ=255)
                                                                                                                     ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCa                                                             <  2:487411/79‑1 (MQ=255)
                                                                                                                                aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag                                                                  >  1:967143/1‑63 (MQ=255)
                                                                                                                                aggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag                                                                  <  2:967143/63‑1 (MQ=255)
                                                                                                                                    |                                                                                                                          
CCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCG  >  NC_000913/911000‑911254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: