Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,072,060 G→A L34L (CTG→TTG)  rutC ← putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,072,0600GA100.0% 143.0 / NA 45L34L (CTG→TTG) rutCputative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/22);  total (23/22)

ATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGG  >  NC_000913/1071927‑1072190
                                                                                                                                     |                                                                                                                                  
aTGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               <  2:351594/139‑1 (MQ=255)
 tGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               <  2:970336/138‑1 (MQ=255)
 tGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               >  1:970336/1‑138 (MQ=255)
              cacaTCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacc                                                                                                                 >  2:836602/1‑139 (MQ=255)
                         tCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagc                                                                                                      <  1:836602/139‑1 (MQ=255)
                             gCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccagc                                                                                                  <  1:31724/139‑1 (MQ=255)
                                 cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGc                                                                                              >  1:1053362/1‑139 (MQ=255)
                                 cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGc                                                                                              >  2:26610/1‑139 (MQ=255)
                                                  cTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGcc                                                                             >  1:1065467/1‑139 (MQ=255)
                                                     gCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATa                                                                                                                     >  2:547004/1‑96 (MQ=255)
                                                     gCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATa                                                                                                                     <  1:547004/96‑1 (MQ=255)
                                                            gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                       >  1:164816/1‑135 (MQ=255)
                                                            gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                       <  2:164816/135‑1 (MQ=255)
                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCg                                                        >  1:546441/1‑139 (MQ=255)
                                                                       gTGGCGGGTTTGCGCCTTTGGGCCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTg                                                                                               >  2:984015/1‑100 (MQ=255)
                                                                       gTGGCGGGTTTGCGCCTTTGGGCCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTg                                                                                               <  1:984015/100‑1 (MQ=255)
                                                                         ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGt                                                                                                                                 <  1:1061843/64‑1 (MQ=255)
                                                                         ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGt                                                                                                                                 >  2:1061843/1‑64 (MQ=255)
                                                                         ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                       >  1:650807/1‑74 (MQ=255)
                                                                         ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                       <  2:650807/74‑1 (MQ=255)
                                                                                             tCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                                  <  2:1053362/139‑1 (MQ=255)
                                                                                              cATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               >  1:872034/1‑45 (MQ=255)
                                                                                              cATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               >  1:33057/1‑45 (MQ=255)
                                                                                              cATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               <  2:872034/45‑1 (MQ=255)
                                                                                              cATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                               <  2:33057/45‑1 (MQ=255)
                                                                                                  ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                           <  2:175606/93‑1 (MQ=255)
                                                                                                  ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                           >  1:175606/1‑93 (MQ=255)
                                                                                                  ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                    <  2:120173/132‑1 (MQ=255)
                                                                                                  ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                    >  1:120173/1‑132 (MQ=255)
                                                                                                     aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGATTAATTACGGATTTTGGCa                                  <  1:885900/131‑1 (MQ=255)
                                                                                                     aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGATTAATTACGGATTTTGGCa                                  >  2:885900/1‑131 (MQ=255)
                                                                                                     aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                          <  1:26610/139‑1 (MQ=255)
                                                                                                        cAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                             >  1:646701/1‑37 (MQ=255)
                                                                                                        cAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                             <  2:646701/37‑1 (MQ=255)
                                                                                                             cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccagc                                                                                                  >  1:215019/1‑59 (MQ=255)
                                                                                                             cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccagc                                                                                                  <  2:215019/59‑1 (MQ=255)
                                                                                                             cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                    <  1:1299821/121‑1 (MQ=255)
                                                                                                             cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                    >  2:1299821/1‑121 (MQ=255)
                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg                                                                                   <  1:790382/61‑1 (MQ=255)
                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg                                                                                   >  2:790382/1‑61 (MQ=255)
                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc                                                                              <  2:505/66‑1 (MQ=255)
                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc                                                                              >  1:505/1‑66 (MQ=255)
                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa     >  2:1160641/1‑139 (MQ=255)
                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa     >  1:594568/1‑139 (MQ=255)
                                                                                                                             aaaaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAgg  <  1:1160641/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
ATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGG  >  NC_000913/1071927‑1072190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: