Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,038	G→A	A325T (GCT→ACT)	dhaR →	dhaKLM operon transcription activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,038	0	G	A	100.0%	138.9 / NA	45	A325T (GCT→ACT)	dhaR	dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/22);  total (23/22)

TTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATT  >  NC_000913/1251906‑1252169                                                                                                                                     |                                                                                                                                    tttATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               <  1:426894/139‑1 (MQ=255)                        tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACg                                                                                                                   <  2:237167/128‑1 (MQ=255)                        tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACg                                                                                                                   >  1:237167/1‑128 (MQ=255)                        tAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAAcc                                                                                                        >  1:836597/1‑139 (MQ=255)                                        aCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTCGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCt                                                                                                 >  2:773061/1‑130 (MQ=255)                                        aCGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCt                                                                                                 <  1:773061/130‑1 (MQ=255)                                            aGGTAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTgg                                                                                    <  2:797719/139‑1 (MQ=255)                                             gggAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACccg                                                                                                      <  1:179950/120‑1 (MQ=255)                                             gggAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACccg                                                                                                      >  2:179950/1‑120 (MQ=255)                                                   cAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGCCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAg                                                                             >  1:476291/1‑139 (MQ=255)                                                    aGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               >  1:288041/1‑88 (MQ=255)                                                    aGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               <  2:288041/88‑1 (MQ=255)                                                        ttATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcggc                                                                        >  2:757883/1‑139 (MQ=255)                                                                 tgCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAg                                                               <  2:20708/139‑1 (MQ=255)                                                                        tCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCGGGCGGCGCGCAGTAGCTTtcct                                                        >  1:472613/1‑139 (MQ=255)                                                                          cTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               >  1:305940/1‑66 (MQ=255)                                                                          cTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               <  2:305940/66‑1 (MQ=255)                                                                             tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCcgccgc                                                                                                  >  1:32021/1‑92 (MQ=255)                                                                             tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCcgccgc                                                                                                  <  2:32021/92‑1 (MQ=255)                                                                             tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                       <  1:1034324/119‑1 (MQ=255)                                                                             tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGgcggcg                                                                       >  2:1034324/1‑119 (MQ=255)                                                                             tgGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcct                                                   >  1:474078/1‑139 (MQ=255)                                                                              ggAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                  >  2:154367/1‑139 (MQ=255)                                                                                  cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               >  1:112643/1‑58 (MQ=255)                                                                                  cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               <  2:112643/58‑1 (MQ=255)                                                                                  cAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCttt                                              >  2:393687/1‑139 (MQ=255)                                                                                     aTGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               <  2:1269911/55‑1 (MQ=255)                                                                                     aTGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCata                                                                                                                               >  1:1269911/1‑55 (MQ=255)                                                                                     aTGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtc                                                     <  2:451945/129‑1 (MQ=255)                                                                                     aTGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtc                                                     >  1:451945/1‑129 (MQ=255)                                                                                           ctgTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGagaag                                     <  2:781906/137‑1 (MQ=255)                                                                                               tgatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                    <  2:783605/104‑1 (MQ=255)                                                                                               tgatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                    <  2:1149222/104‑1 (MQ=255)                                                                                               tgatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                    >  1:1149222/1‑104 (MQ=255)                                                                                               tgatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGgcgcgc                                                                    >  1:783605/1‑104 (MQ=255)                                                                                                gatgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCCTTGTGGAGAAGAGGGc                                <  1:757883/139‑1 (MQ=255)                                                                                                        gTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGt                              <  2:1218918/133‑1 (MQ=255)                                                                                                        gTCAATTAGGAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGt                              >  1:1218918/1‑133 (MQ=255)                                                                                                         tCAATTAGGAAAAGCCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAg                       <  2:476291/139‑1 (MQ=255)                                                                                                                ggAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcgg                                                                         >  2:32329/1‑82 (MQ=255)                                                                                                                ggAAAAGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcgg                                                                         <  1:32329/82‑1 (MQ=255)                                                                                                                     aGTCAGCCATACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGt           >  1:439708/1‑139 (MQ=255)                                                                                                                     aGTCAGCCATACCTTCACTCATATGCCACAGGACGAT‑CGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                  <  2:1012789/99‑1 (MQ=255)                                                                                                                     aGTCAGCCATACCTTCACTCATATGCCACAGGACGAT‑CGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTtcctgtcctg                                                  >  1:1012789/1‑99 (MQ=255)                                                                                                                              tACCTTCACTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAAtt  <  1:393687/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    TTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCATATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATT  >  NC_000913/1251906‑1252169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑