Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,260,528 | A→G | I92I (ATT→ATC) | dauA ← | C4‑dicarboxylic acid transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,260,528 | 0 | A | G | 97.2% | 122.5 / ‑6.2 | 36 | I92I (ATT→ATC) | dauA | C4‑dicarboxylic acid transporter |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/16); minor base C (0/1); total (19/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAA > NC_000913/1260410‑1260649 | gAATATACTCAATCAGGCGACCAAAGCGTGCCAGACGCATCAGAATCAAAAAGATCCCCGACTGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGt < 2:964193/139‑1 (MQ=255) tCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCgg > 2:970861/1‑139 (MQ=255) ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa < 2:723710/134‑1 (MQ=255) ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGaa > 1:723710/1‑134 (MQ=255) ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt > 2:601125/1‑139 (MQ=255) ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCt > 1:1099174/1‑139 (MQ=255) gCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTa < 1:709432/139‑1 (MQ=255) acgaaaGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCCGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAg < 2:966420/136‑1 (MQ=255) ccAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCg < 2:693571/115‑1 (MQ=255) ccAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCg > 1:693571/1‑115 (MQ=255) ccAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGc > 1:897072/1‑139 (MQ=255) cccattaggaataaaaaaATCCCCGGAAGCAAGGGCGCAACCAGCAGTCCTGCCCGTTCCAAACTTTGCCGAAACGGGTAGGGGATTTCCCCCAATTGCCG‑AGGCGGACCGGGAACGCCAAAACGGGACCCAACCGACa > 2:1287178/13‑139 (MQ=255) cAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCa > 1:969113/1‑139 (MQ=255) cAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa < 2:969113/139‑1 (MQ=255) aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt > 1:1260551/1‑119 (MQ=255) aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt > 2:665233/1‑119 (MQ=255) aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt < 1:665233/119‑1 (MQ=255) aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt < 2:1260551/119‑1 (MQ=255) aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCg > 1:663593/1‑139 (MQ=255) gCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag > 1:582153/1‑114 (MQ=255) gCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag < 2:582153/114‑1 (MQ=255) gCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGac > 2:139114/1‑93 (MQ=255) gCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGac < 1:139114/93‑1 (MQ=255) cAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca > 2:89587/1‑95 (MQ=255) cAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca < 1:89587/95‑1 (MQ=255) cagTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGa > 1:782130/1‑69 (MQ=255) cagTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGCATTACCACAAATGCCGCAGTCGGACCAGa < 2:782130/69‑1 (MQ=255) ccAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCaa > 2:482502/1‑108 (MQ=255) ccAG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCaa < 1:482502/108‑1 (MQ=255) aG‑TCCAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCca < 1:1145549/139‑1 (MQ=255) ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg < 2:1217242/128‑1 (MQ=255) ccAAACTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTg > 1:1217242/1‑128 (MQ=255) aaCTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCAcaccac < 2:663593/139‑1 (MQ=255) aaCTGTTGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCAcaccac > 1:1162164/1‑139 (MQ=255) ttGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg > 2:675816/1‑60 (MQ=255) ttGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCg < 1:675816/60‑1 (MQ=255) tGCGACACGGGATAGAGGATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCaa > 1:777693/1‑139 (MQ=255) | GAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAG‑TCCAAACTGTTGCGACACGGGATAGAGAATTACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAA > NC_000913/1260410‑1260649 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |