Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,095	G→A	A126T (GCA→ACA)	tyrR →	aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,095	0	G	A	100.0%	50.2 / NA	17	A126T (GCA→ACA)	tyrR	aromatic amino acid biosynthesis and transport regulon transcriptional regulator, autorepressor, ATPase, phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

GGCGTTGAGCGCGTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCGCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGAAAA  >  NC_000913/1386959‑1387228                                                                                                                                         |                                                                                                                                      ggCGTTGAGCGCGTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCcaca                                                                                                                                     >  2:600476/1‑139 (MQ=255)                           tGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAAtttttt                                                                                                           >  1:1313720/1‑139 (MQ=255)                           tGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAAtttttt                                                                                                           >  2:1326311/1‑139 (MQ=255)                                       tgCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                       >  1:1191063/1‑131 (MQ=255)                                       tgCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGt                                                                                                       <  2:1191063/131‑1 (MQ=255)                                                                aGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCAt                                                                         <  1:1251082/136‑1 (MQ=255)                                                                aGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCAt                                                                         >  2:1251082/1‑136 (MQ=255)                                                                              aCCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCttt                                                                                                                   >  1:947071/1‑80 (MQ=255)                                                                              aCCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCttt                                                                                                                   <  2:947071/80‑1 (MQ=255)                                                                              aCCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGttat                                                        >  1:83157/1‑139 (MQ=255)                                                                                     gAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATggg                                                 <  1:600476/139‑1 (MQ=255)                                                                                          gTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGaa                                            <  1:1326311/139‑1 (MQ=255)                                                                                                    tGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATg                                  <  2:1313720/139‑1 (MQ=255)                                                                                                     gggCAAAAATTGGATCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATgg                                 >  2:664691/1‑139 (MQ=255)                                                                                                             aTTGGATCGCCTTCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAAGGGACAGAATTTCCTGATGGAGATTACg                         <  2:803597/139‑1 (MQ=255)                                                                                                                ggAGCGCCTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTATGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCGGATGGAGATTACGCCt                      >  1:915705/1‑139 (MQ=255)                                                                                                                      ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCg                                                                            <  1:538773/79‑1 (MQ=255)                                                                                                                      ccTGCGCAACCATACCGCCACACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCg                                                                            >  2:538773/1‑79 (MQ=255)                                                                                                                                    ccgccaCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGaaaa  >  1:815015/1‑139 (MQ=255)                                                                                                                                    ccgccaCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGaaaa  >  2:427723/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      GGCGTTGAGCGCGTTACTGGAGGCGTTGCCTGAACCTGTGCTCTCTGTCGATATGAAAAGCAAAGTGGATATGGCGAACCCGGCGAGCTGTCAGCTTTTTGGGCAAAAATTGGATCGCCTGCGCAACCATACCGCCGCACAATTGATTAACGGCTTTAATTTTTTACGTTGGCTGGAAAGCGAACCGCAAGATTCGCATAACGAGCATGTCGTTATTAATGGGCAGAATTTCCTGATGGAGATTACGCCTGTTTATCTTCAGGATGAAAA  >  NC_000913/1386959‑1387228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑