Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	122,856	G→A	*255* (TAG→TAA)	pdhR →	pyruvate dehydrogenase complex repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	122,856	0	G	A	100.0%	150.9 / NA	48	*255* (TAG→TAA)	pdhR	pyruvate dehydrogenase complex repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/27);  total (21/27)

GTAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACG  >  NC_000913/122726‑122991                                                                                                                                   |                                                                                                                                        gTAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGAttttt                                                                                                                                 <  1:124329/139‑1 (MQ=255)  tAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTc                                                                                                                                >  2:175661/1‑139 (MQ=255)  tAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTAGCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTc                                                                                                                                >  2:283351/1‑139 (MQ=255)           agaagCGCGCGAAGCATCGCATCGCGATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTaaaaa                                                                                                                       <  1:692720/139‑1 (MQ=255)               gcgcgcGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTAt                                                                                                                   <  1:1156701/139‑1 (MQ=255)                                 cgcCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGAttttt                                                                                                                                 >  2:475488/1‑107 (MQ=255)                                 cgcCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGAttttt                                                                                                                                 <  1:475488/107‑1 (MQ=255)                                      tCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                            <  1:175661/139‑1 (MQ=255)                                      tCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                            <  1:283351/139‑1 (MQ=255)                                      tCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc                                                                                            <  2:1344075/139‑1 (MQ=255)                                              ttATCGAAGAAATTTTGCACGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCaga                                                                                                              >  2:864855/1‑113 (MQ=255)                                              ttATCGAAGAAATTTTGCACGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCaga                                                                                                              <  1:864855/113‑1 (MQ=255)                                                    aagaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAgcgc                                                                              >  1:997550/1‑139 (MQ=255)                                                      gaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCgg                                                                            <  2:495842/139‑1 (MQ=255)                                                      gaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCgg                                                                            <  2:345034/139‑1 (MQ=255)                                                                         gTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTg                                                         <  2:997550/139‑1 (MQ=255)                                                                         gTCGTGAAGAGAGCCGCCGTGAGCGTTCTATGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGACGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTg                                                         <  1:909949/139‑1 (MQ=255)                                                                              gAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTc                                                       <  2:206942/136‑1 (MQ=255)                                                                              gAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTc                                                       >  1:206942/1‑136 (MQ=255)                                                                                    agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGc                                              >  1:943918/1‑139 (MQ=255)                                                                                        gccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTc                                          >  2:351437/1‑139 (MQ=255)                                                                                         ccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTcc                                         >  1:383025/1‑139 (MQ=255)                                                                                            tGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGc                                      <  1:351437/139‑1 (MQ=255)                                                                                                 gTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGagag                                 <  2:383025/139‑1 (MQ=255)                                                                                                   tctcTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAATGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGtt                               >  2:897409/1‑139 (MQ=255)                                                                                                      ctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGATCTTTCCGGCGAGAGTTCaa                            <  1:897409/139‑1 (MQ=255)                                                                                                       tGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAAc                                                            >  2:719088/1‑106 (MQ=255)                                                                                                       tGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAAc                                                            <  1:719088/106‑1 (MQ=255)                                                                                                         cgtcgtCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGAtgttg                                                                                                      <  1:407933/62‑1 (MQ=255)                                                                                                         cgtcgtCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGAtgttg                                                                                                      >  2:407933/1‑62 (MQ=255)                                                                                                         cgtcgtCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATgg                         >  2:94894/1‑139 (MQ=255)                                                                                                           tcgtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGa                       <  2:943918/139‑1 (MQ=255)                                                                                                           tcgtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGa                       <  2:1190409/139‑1 (MQ=255)                                                                                                             gtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGc                                      >  1:665296/1‑122 (MQ=255)                                                                                                             gtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGc                                      <  2:665296/122‑1 (MQ=255)                                                                                                              tcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAg                    >  1:734083/1‑139 (MQ=255)                                                                                                              tcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAg                    >  2:859825/1‑139 (MQ=255)                                                                                                                 ggAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGtt                 >  1:919261/1‑139 (MQ=255)                                                                                                                   aGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCGTATAAAAGCGCGGCAACTAAACGTAGa                                                              <  2:1261943/92‑1 (MQ=255)                                                                                                                   aGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCGTATAAAAGCGCGGCAACTAAACGTAGa                                                              >  1:1261943/1‑92 (MQ=255)                                                                                                                        cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACt                                                                       >  1:463289/1‑78 (MQ=255)                                                                                                                        cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACt                                                                       <  2:463289/78‑1 (MQ=255)                                                                                                                        cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGc                                      <  1:391391/111‑1 (MQ=255)                                                                                                                        cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGc                                      >  2:391391/1‑111 (MQ=255)                                                                                                                        cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGaaa          >  1:1284029/1‑139 (MQ=255)                                                                                                                              aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa    <  1:859825/139‑1 (MQ=255)                                                                                                                              aaTTAATGATTATTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa    <  1:94894/139‑1 (MQ=255)                                                                                                                                ttAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACg  <  2:1284029/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GTAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACG  >  NC_000913/122726‑122991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑