| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,227 | T→C | R737R (CGT→CGC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,227 | 0 | T | C | 100.0% | 76.7 / NA | 22 | R737R (CGT→CGC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/12); total (10/12) | |||||||||||
AACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGC > NC_000913/125096‑125357 | aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTcc > 1:1070048/1‑139 (MQ=255) aaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag < 1:888255/139‑1 (MQ=255) aCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagcagc > 1:248254/1‑139 (MQ=255) gCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATc < 2:1070048/139‑1 (MQ=255) gCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTgg < 2:703895/139‑1 (MQ=255) aaTGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCg < 2:935926/139‑1 (MQ=255) ggCCGGAAGGTGCTGCGGAACGAATCCGTAAAGGTACCTACAAACTCGAAACTATTTAAGGAAGCCAAGGTAAAGTTCCGCTCCTCGGCTCCGGTTCTATCCTGCGCCACGTCCATGCAGCAGCTGAGATCCGGGCaaa > 2:842689/2‑139 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGa > 1:589792/1‑118 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGa < 2:589792/118‑1 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGt < 1:1295424/131‑1 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGt > 2:1295424/1‑131 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTg > 2:1013779/1‑139 (MQ=255) tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg < 1:1229498/139‑1 (MQ=255) aaaCTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAcctcct > 1:1229131/1‑139 (MQ=255) cGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCAcc > 2:1196360/1‑139 (MQ=255) aaCTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAg < 1:1013779/139‑1 (MQ=255) ggTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg < 2:1229131/139‑1 (MQ=255) cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt < 1:1196360/139‑1 (MQ=255) gctgctCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGaa < 2:1188051/60‑1 (MQ=255) gctgctCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGaa > 1:1188051/1‑60 (MQ=255) tCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGaa < 2:248254/139‑1 (MQ=255) aTCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGc > 2:821662/1‑139 (MQ=255) | AACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGCTGGAACATGCTGCACCCGC > NC_000913/125096‑125357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |