Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,052	G→A	*263* (TAG→TAA)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,052	0	G	A	100.0%	114.9 / NA	38	*263* (TAG→TAA)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

ACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCG  >  NC_000913/1458924‑1459183                                                                                                                                 |                                                                                                                                    aCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGTCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATa                                                                                                                           <  1:644954/139‑1 (MQ=255)                       tGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGa                                                                                                     >  1:167231/1‑139 (MQ=255)                              ggAACGTGACTATCAGCGACTTGCCGGACGGAGCACAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                         >  2:82333/1‑128 (MQ=255)                              ggAACGTGACTATCAGCGACTTGCCGGACGGAGCACAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                         <  1:82333/128‑1 (MQ=255)                                aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCg                                                                                            >  2:688620/1‑139 (MQ=255)                                 aCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                           <  1:824524/139‑1 (MQ=255)                                   gTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCa                                                                                         >  1:675851/1‑139 (MQ=255)                                   gTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCACAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCa                                                                                         >  2:965460/1‑139 (MQ=255)                                    tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                              <  1:509416/133‑1 (MQ=255)                                    tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                              >  2:509416/1‑133 (MQ=255)                                    tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc                                                                                        >  1:52188/1‑139 (MQ=255)                                          tCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCGTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATggg                                                                                  >  1:908690/1‑139 (MQ=255)                                                           ggaGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAg                                                                 <  1:965460/139‑1 (MQ=255)                                                             aGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACatgat                                                                                                                               >  1:341833/1‑75 (MQ=255)                                                             aGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACatgat                                                                                                                               <  2:341833/75‑1 (MQ=255)                                                                  aGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                          <  1:688620/139‑1 (MQ=255)                                                                  aGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                          <  2:167231/139‑1 (MQ=255)                                                                             aaGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgtg                                                                                                                     <  2:624675/69‑1 (MQ=255)                                                                             aaGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgtg                                                                                                                     >  1:624675/1‑69 (MQ=255)                                                                              aGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                              >  2:21366/1‑139 (MQ=255)                                                                                gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACAcc                                            <  1:726719/139‑1 (MQ=255)                                                                                  gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                           <  2:891742/90‑1 (MQ=255)                                                                                  gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                           >  1:891742/1‑90 (MQ=255)                                                                                   tCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAgg                                         <  1:1173246/139‑1 (MQ=255)                                                                                       tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGtttt                                     >  2:1129659/1‑139 (MQ=255)                                                                                       tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGtttt                                     <  1:1129659/139‑1 (MQ=255)                                                                                                    aaaCGCTCACCGCAGTTCTCGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACAtt                        <  1:904780/139‑1 (MQ=255)                                                                                                      aCGCTCACCGCAGTTCACGGGGAAAGAAGATGATGGTAGGTCTGCAAACTGTGGCAGTGATTGGGAGCGGCACGAGGGCGGCAGGCCTTGCTGAAGTTGCTGCCAATCATGGACACGAGGTTTTACTGTACTACAtttg                      >  2:853529/1‑138 (MQ=255)                                                                                                           cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTgg                                                                                                           <  1:852063/49‑1 (MQ=255)                                                                                                           cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTgg                                                                                                           >  2:852063/1‑49 (MQ=255)                                                                                                           cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGTCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                 >  2:805465/1‑139 (MQ=255)                                                                                                           cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                 <  2:675851/139‑1 (MQ=255)                                                                                                           cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                 <  1:226808/139‑1 (MQ=255)                                                                                                           cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                 <  2:908690/139‑1 (MQ=255)                                                                                                                          ggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTAt                              >  2:677037/1‑111 (MQ=255)                                                                                                                          ggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTAt                              <  1:677037/111‑1 (MQ=255)                                                                                                                          ggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGCCCcgcg  <  1:805465/139‑1 (MQ=255)                                                                                                                          ggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCcgcg  <  2:52188/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    ACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCG  >  NC_000913/1458924‑1459183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑