Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,464,489 G→A *317* (TAG→TAA
D7N (GAC→AAC) 
paaX →
paaY →
transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
thioesterase required for phenylacetic acid degradation, trimeric, phenylacetate regulatory and detoxification protein, hexapeptide repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,464,4890GA95.2% 57.5 / ‑5.8 21*317* (TAG→TAA
D7N (GAC→AAC) 
paaX
paaY
transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
thioesterase required for phenylacetic acid degradation, trimeric, phenylacetate regulatory and detoxification protein, hexapeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (10/10);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCG  >  NC_000913/1464356‑1464621
                                                                                                                                     |                                                                                                                                    
ctgctgCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGt                                                                                                                                 <  1:209003/139‑1 (MQ=255)
     gctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGAc                                                                                                                            <  2:533518/139‑1 (MQ=255)
             gttcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTtg                                                                                                                    <  1:487227/139‑1 (MQ=255)
                cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGc                                                                                                                 >  2:1192492/1‑139 (MQ=255)
                                        ggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGTCAGAAGAGAGTTTTGTTCATCCGa                                                                                         <  1:822690/139‑1 (MQ=255)
                                                    gccggcGCTGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATCGAACAGGGGGTGTTATGCTAATTTATGATATAGACGGTCTGTCTCCGGTTGTGCCGGTAGAGAGGTTTGTCCATCCGACAGCGGTATTgg                                                                             >  2:944496/1‑138 (MQ=255)
                                                        gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                         >  1:781588/1‑139 (MQ=255)
                                                               gAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGtt                                                                  >  1:1000275/1‑139 (MQ=255)
                                                                    cTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATtct                                                             <  2:1000275/139‑1 (MQ=255)
                                                                                      ggcggcTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGATCTGACTCCGGTTGTGCCAGACGATAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGAAAGGGCGTTTACGt                                           >  1:825329/1‑139 (MQ=255)
                                                                                            ttGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                         >  1:1300338/1‑87 (MQ=255)
                                                                                            ttGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                         <  2:1300338/87‑1 (MQ=255)
                                                                                                        cAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                >  1:282936/1‑68 (MQ=255)
                                                                                                        cAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                <  2:282936/68‑1 (MQ=255)
                                                                                                             ggCGTTATGCCAATTTATCAGATAAACGGTCTGACt                                                                                                                           >  2:954406/1‑36 (MQ=255)
                                                                                                             ggCGTTATGCCAATTTATCAGATAAACGGTCTGACt                                                                                                                           <  1:954406/36‑1 (MQ=255)
                                                                                                                     gCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAtttt            >  1:763838/1‑139 (MQ=255)
                                                                                                                          tttATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGtt                                                                                                     >  2:1254658/1‑45 (MQ=255)
                                                                                                                          tttATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGtt                                                                                                     <  1:1254658/45‑1 (MQ=255)
                                                                                                                           ttATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGt      >  1:526877/1‑139 (MQ=255)
                                                                                                                               cAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg  <  2:781588/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCG  >  NC_000913/1464356‑1464621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: