Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,467,921 C→T *302* (TAG→TAA insD1 ← IS2 transposase TnpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,467,9210CT100.0% 101.8 / NA 34*302* (TAG→TAAinsD1IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

CTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT  >  NC_000913/1467793‑1468042
                                                                                                                                |                                                                                                                         
ctctGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCa                                                                                                                 >  1:1104593/1‑139 (MQ=255)
ctctGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCa                                                                                                                 <  2:1104593/139‑1 (MQ=255)
                   tATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTaa                                                                                              <  2:438544/139‑1 (MQ=255)
                                  tAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTc                                                                                                                   >  2:410172/1‑103 (MQ=255)
                                  tAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTc                                                                                                                   <  1:410172/103‑1 (MQ=255)
                                          tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCa                                                                       >  1:1252277/1‑139 (MQ=255)
                                                 aaTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                            <  2:534560/111‑1 (MQ=255)
                                                 aaTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                            >  1:534560/1‑111 (MQ=255)
                                                            gcgcTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                          >  2:847508/1‑134 (MQ=255)
                                                            gcgcTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                          <  1:847508/134‑1 (MQ=255)
                                                              gctacgctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcga                                                   <  2:1252277/139‑1 (MQ=255)
                                                                tacgctgACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGt                                                                                                        <  2:852954/84‑1 (MQ=255)
                                                                tacgctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGt                                                                                                        >  1:852954/1‑84 (MQ=255)
                                                                      aaCAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAg                                           >  1:476218/1‑139 (MQ=255)
                                                                        cAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                         <  2:476218/139‑1 (MQ=255)
                                                                            gCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACt                                     >  1:1047900/1‑139 (MQ=255)
                                                                                   atTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               >  2:182159/1‑90 (MQ=255)
                                                                                   atTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               <  1:182159/90‑1 (MQ=255)
                                                                                     tGACAGTTTTGAACATCATGGGACATGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                         >  2:144337/1‑126 (MQ=255)
                                                                                     tGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGGCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                         <  1:144337/126‑1 (MQ=255)
                                                                                             ttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa                                                                                                               <  1:776786/48‑1 (MQ=255)
                                                                                             ttGAACATCATGGGACAAGAACCCTGGATATGCCCTTATATTTCCAGa                                                                                                               >  2:776786/1‑48 (MQ=255)
                                                                                              tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa                                                                                                               >  2:1142983/1‑47 (MQ=255)
                                                                                              tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa                                                                                                               <  1:1142983/47‑1 (MQ=255)
                                                                                              tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCa                                            >  2:1012766/1‑114 (MQ=255)
                                                                                              tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCGGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCa                                            <  1:1012766/114‑1 (MQ=255)
                                                                                                     catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 >  2:25239/1‑54 (MQ=255)
                                                                                                     catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 <  1:25239/54‑1 (MQ=255)
                                                                                                          gACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa       <  1:55849/139‑1 (MQ=38)
                                                                                                               gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                      <  1:887376/39‑1 (MQ=255)
                                                                                                               gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                      >  2:887376/1‑39 (MQ=14)
                                                                                                               gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 <  1:902583/44‑1 (MQ=255)
                                                                                                               gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct  >  1:287212/1‑139 (MQ=18)
                                                                                                               gAACCCTGGATTTACCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 >  2:902583/1‑44 (MQ=14)
                                                                                                                                |                                                                                                                         
CTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT  >  NC_000913/1467793‑1468042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: