| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,493,219 | A→G | E250E (GAA→GAG) | trg → | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,493,219 | 0 | A | G | 97.1% | 116.6 / NA | 35 | E250E (GAA→GAG) | trg | methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (16/18); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGC > NC_000913/1493085‑1493350 | ggCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg < 2:1052368/139‑1 (MQ=255) gcgcGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcggc > 2:350854/1‑139 (MQ=255) cgTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCggg < 1:223725/139‑1 (MQ=255) ccTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATc > 1:239977/1‑139 (MQ=255) ccTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATc > 1:413576/1‑139 (MQ=255) ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg > 1:1014771/1‑119 (MQ=255) ggTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCcgg < 2:1014771/119‑1 (MQ=255) gACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTa > 2:162523/1‑139 (MQ=255) cGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg < 2:944522/139‑1 (MQ=255) cGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAg < 2:413576/139‑1 (MQ=255) tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcg > 2:1068914/1‑139 (MQ=255) tGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtcg > 2:1040995/1‑139 (MQ=255) cATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCAttt < 1:350854/139‑1 (MQ=255) gCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATg > 2:1307903/1‑139 (MQ=255) cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtc < 2:1020699/121‑1 (MQ=255) cTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAgtc > 1:1020699/1‑121 (MQ=255) cGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTg < 2:151017/139‑1 (MQ=255) tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca < 1:1088557/118‑1 (MQ=255) tGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATgcagca > 2:1088557/1‑118 (MQ=255) cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGa < 1:162523/139‑1 (MQ=255) cAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCAGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGa < 2:239977/139‑1 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCaa < 1:1199541/100‑1 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCaa > 2:1199541/1‑100 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGa < 1:567381/119‑1 (MQ=255) cGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGa > 2:567381/1‑119 (MQ=255) cGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGgcg > 1:1131039/1‑139 (MQ=255) aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt < 2:404186/92‑1 (MQ=255) aTTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAAt > 1:404186/1‑92 (MQ=255) ttGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGa < 1:1307903/139‑1 (MQ=255) tGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGaa < 2:1131039/139‑1 (MQ=255) aTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg > 1:1194359/1‑102 (MQ=255) aTCGCCAGTGGCGATCTGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATg < 2:1194359/102‑1 (MQ=255) tGACGATGAATGATGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGa < 1:1068914/139‑1 (MQ=255) aatgaTGAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTAACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAg > 1:648904/1‑139 (MQ=255) atgatgAGCCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGc > 2:679595/1‑139 (MQ=255) | GGCGCGTTTGTGCTTGCCCTGGTCATGACGCTGATAACATTTATGGTGCTACGTCGGATCGTCATTCGTCCACTGCAACATGCCGCACAACGGATTGAAAAAATCGCCAGTGGCGATCTGACGATGAATGATGAACCGGCGGGTCGTAATGAAATCGGTCGCTTAAGTCGTCATTTACAGCAAATGCAGCATTCACTGGGGATGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGC > NC_000913/1493085‑1493350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |