Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 152,036 C→T V66I (GTA→ATA)  yadL ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913152,0360CT100.0% 158.2 / NA 50V66I (GTA→ATA) yadLputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/26);  total (24/26)

TTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACG  >  NC_000913/151910‑152163
                                                                                                                              |                                                                                                                               
ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     >  2:636806/1‑139 (MQ=255)
ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     <  1:1283254/139‑1 (MQ=255)
ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     <  1:636806/139‑1 (MQ=255)
  cccTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaa                                                                                                                   >  2:629770/1‑139 (MQ=255)
            tgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAAt                                                                                                         >  2:66858/1‑139 (MQ=255)
                      ggTTAATTTTATTTGCGCTTTTTTCTTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCa                                                                                               <  1:750671/139‑1 (MQ=255)
                                       cTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGt                                                                              <  1:78287/139‑1 (MQ=255)
                                         tttttATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAg                                                                            <  1:336177/139‑1 (MQ=255)
                                                tGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  2:173242/1‑101 (MQ=255)
                                                tGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:173242/101‑1 (MQ=255)
                                                  gggATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  2:901448/99‑1 (MQ=255)
                                                  gggATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  1:901448/1‑99 (MQ=255)
                                                    gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAGTCAACAACAGaa                                                                                                           >  2:886036/1‑97 (MQ=255)
                                                    gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:886036/97‑1 (MQ=255)
                                                    gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAg                                                                            >  2:65281/1‑128 (MQ=255)
                                                    gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAg                                                                            <  1:65281/128‑1 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     <  1:420039/74‑1 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     >  2:420039/1‑74 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  2:564216/84‑1 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  1:564216/1‑84 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCagag                                                                                            <  2:559071/99‑1 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCagag                                                                                            >  1:559071/1‑99 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                         >  2:324923/1‑102 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                         <  1:324923/102‑1 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                      >  2:552179/1‑137 (MQ=255)
                                                                 tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                      <  1:552179/137‑1 (MQ=255)
                                                                   tttcaaTTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGc                                                                           >  1:1256584/5‑114 (MQ=255)
                                                                   tttCAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGc                                                                           <  2:1256584/114‑1 (MQ=255)
                                                                           tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCt                                                        >  2:717457/1‑125 (MQ=255)
                                                                           tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCt                                                        <  1:717457/125‑1 (MQ=255)
                                                                             aGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAATAGaa                                                                                                           >  1:839055/1‑72 (MQ=255)
                                                                             aGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGag                                                                                                           <  2:839055/72‑2 (MQ=255)
                                                                             aGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACgg                                        >  2:297414/1‑139 (MQ=255)
                                                                              gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       <  1:297414/139‑1 (MQ=255)
                                                                                   aaTGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTc                                  <  1:629770/139‑1 (MQ=255)
                                                                                         tttACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                 >  1:957209/1‑102 (MQ=255)
                                                                                         tttACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                 <  2:957209/102‑1 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                         >  2:1102740/1‑70 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                         <  1:1102740/70‑1 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       >  2:187862/1‑72 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       <  1:187862/72‑1 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                       >  1:95400/1‑104 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                       <  2:95400/104‑1 (MQ=255)
                                                                                                 ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGtt                    >  1:1000472/1‑139 (MQ=255)
                                                                                                       ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       >  2:1317541/1‑114 (MQ=255)
                                                                                                       ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                       <  1:1317541/114‑1 (MQ=255)
                                                                                                             gATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagag        >  2:1047425/1‑139 (MQ=255)
                                                                                                                  cAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       >  2:486312/1‑55 (MQ=255)
                                                                                                                  cAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       <  1:486312/55‑1 (MQ=255)
                                                                                                                   aGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACg  <  1:1047425/139‑1 (MQ=255)
                                                                                                                        tatatataTCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                      >  2:865963/1‑82 (MQ=255)
                                                                                                                        tatatataTCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                      <  1:865963/82‑1 (MQ=255)
                                                                                                                              |                                                                                                                               
TTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACG  >  NC_000913/151910‑152163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑