Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,825	C→T	V186I (GTT→ATT)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,825	0	C	T	100.0%	113.8 / NA	36	V186I (GTT→ATT)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

CGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACG  >  NC_000913/1626697‑1626957                                                                                                                                 |                                                                                                                                     cGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgccg                                                                                                                            <  1:271656/139‑1 (MQ=255)           gtggtATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtg                                                                                                                  <  1:468418/139‑1 (MQ=255)             ggtATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgct                                                                                                                >  1:1040380/1‑139 (MQ=255)                   cagcagCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGt                                                                                                          <  1:530956/139‑1 (MQ=255)                         cGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGc                                                                                                    >  1:548911/1‑139 (MQ=255)                         cGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCAAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGc                                                                                                    >  2:795939/1‑139 (MQ=255)                              tCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgc                                                                                                                              <  1:703629/108‑1 (MQ=255)                              tCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGAccgc                                                                                                                              >  2:703629/1‑108 (MQ=255)                                aGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTaa                                                                                             <  1:1146094/139‑1 (MQ=255)                                     tttGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGGAATCGCTGGTTAAActgg                                                                                        <  1:885180/139‑1 (MQ=255)                                      ttGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgctgc                                                                                                              <  1:220764/116‑1 (MQ=255)                                      ttGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgctgc                                                                                                              >  2:220764/1‑116 (MQ=255)                                        gTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAActggctg                                                                                     >  2:507760/1‑139 (MQ=255)                                              cAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAgg                                                                               <  1:507760/139‑1 (MQ=255)                                               aGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTGATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAggg                                                                              >  2:805940/1‑139 (MQ=255)                                                 aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGt                                                                                                          >  1:152097/1‑109 (MQ=255)                                                 aCCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGt                                                                                                          <  2:152097/109‑1 (MQ=255)                                                   cTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTTCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                          <  1:224660/139‑1 (MQ=255)                                                   cTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGc                                                                          <  2:1040380/139‑1 (MQ=255)                                                      tctcGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                            >  2:996584/1‑134 (MQ=255)                                                      tctcGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTc                                                                            <  1:996584/134‑1 (MQ=255)                                                          gcgAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCt                                                                   <  1:795939/139‑1 (MQ=255)                                                               ccgccTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTAtt                                                              <  2:548911/139‑1 (MQ=255)                                                                              gcgcAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAAttt                                               <  2:1283356/139‑1 (MQ=255)                                                                                              actcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgctg                                                                                                               <  2:1162836/59‑1 (MQ=255)                                                                                              actcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTtgctg                                                                                                               >  1:1162836/1‑59 (MQ=255)                                                                                               ctcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTgcg                              <  1:404457/139‑1 (MQ=255)                                                                                                tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTgcg                              <  1:711759/138‑1 (MQ=255)                                                                                                tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTgcg                              >  2:711759/1‑138 (MQ=255)                                                                                                     ccTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGtt                        >  1:544322/1‑139 (MQ=255)                                                                                                                cgcgATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                        >  2:1211104/1‑80 (MQ=255)                                                                                                                cgcgATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                        <  1:1211104/80‑1 (MQ=255)                                                                                                                          ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCt                                                                   >  2:27857/1‑75 (MQ=255)                                                                                                                          ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCt                                                                   <  1:27857/75‑1 (MQ=255)                                                                                                                          ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAAc   >  2:1184228/1‑139 (MQ=255)                                                                                                                           tCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACg  <  1:1184228/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     CGGTTGTTGGGTGGTATTCAGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACAAAACG  >  NC_000913/1626697‑1626957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑