Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,627,625	T→C	L37L (TTG→CTG)	ydfG →	NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,627,625	0	T	C	100.0%	272.0 / NA	76	L37L (TTG→CTG)	ydfG	NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/38);  total (38/38)

ACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAA  >  NC_000913/1627493‑1627737                                                                                                                                     |                                                                                                                 acaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAgg                                                                                                            >  1:505549/1‑139 (MQ=255)        cGTTGAGGAGCAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAAAAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACCGCTGCAGGAGTTaaa                                                                                                     >  1:901949/1‑139 (MQ=255)           tGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGa                                                                                                  >  1:508605/1‑139 (MQ=255)           cgAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGa                                                                                                  >  2:841239/2‑139 (MQ=255)             aGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                <  2:1081958/139‑1 (MQ=255)             aGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                <  2:741560/139‑1 (MQ=255)             aGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                >  1:630188/1‑139 (MQ=255)                     tGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGga                                                                                        >  1:1028868/1‑139 (MQ=255)                       agaTGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgaga                                                                                      >  1:559744/1‑139 (MQ=255)                        gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCTACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGCCGCACTGGGAGAt                                                                                     <  2:901949/139‑1 (MQ=255)                        gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                     <  2:630188/139‑1 (MQ=255)                        gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                     <  2:683193/139‑1 (MQ=255)                        gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                     <  2:134126/139‑1 (MQ=255)                        gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                     <  2:901745/139‑1 (MQ=255)                        gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                     <  2:1028868/139‑1 (MQ=255)                            atCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATc                                                                                 <  1:841239/139‑1 (MQ=255)                            atCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATc                                                                                 >  2:987455/1‑139 (MQ=255)                             tCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACt                                                                                            <  2:669486/127‑1 (MQ=255)                             tCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACt                                                                                            >  1:669486/1‑127 (MQ=255)                             tCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCt                                                                                >  1:27761/1‑139 (MQ=255)                               gTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGt                                                                              >  2:1290518/1‑139 (MQ=255)                                     gTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCg                                                                        <  1:987455/139‑1 (MQ=255)                                      tAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGc                                                                       <  1:1290518/139‑1 (MQ=255)                                      tAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGc                                                                       >  2:179225/1‑139 (MQ=255)                                                  cGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGaa                                                                                              <  2:815985/104‑1 (MQ=255)                                                  cGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGaa                                                                                              >  1:815985/1‑104 (MQ=255)                                                  cGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGtata                                                                           >  2:164275/1‑123 (MQ=255)                                                  cGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGtata                                                                           <  1:164275/123‑1 (MQ=255)                                                              gTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                               <  2:559744/139‑1 (MQ=255)                                                                   tGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTg                                                                                                                >  2:1166552/1‑69 (MQ=255)                                                                             gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                             <  1:813918/62‑1 (MQ=255)                                                                             gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                             >  2:813918/1‑62 (MQ=255)                                                                             gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGAt                                >  1:1216418/1‑139 (MQ=255)                                                                             gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGAt                                >  2:563739/1‑139 (MQ=255)                                                                             gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGAt                                >  1:1006318/1‑139 (MQ=255)                                                                              tcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                       <  2:223399/83‑1 (MQ=255)                                                                              tcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                       >  1:223399/1‑83 (MQ=255)                                                                               cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                             >  1:144861/1‑124 (MQ=255)                                                                               cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                             <  2:144861/124‑1 (MQ=255)                                                                               cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGc                              >  1:925062/1‑139 (MQ=255)                                                                                gtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGta                                                                             >  1:1076414/1‑91 (MQ=255)                                                                                gtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGta                                                                             <  2:1076414/91‑1 (MQ=255)                                                                                   ttATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAaga                                   <  2:1010909/130‑1 (MQ=255)                                                                                   ttATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAaga                                   >  1:1010909/1‑130 (MQ=255)                                                                                    tATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGGCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGGATGTCGCCTAACTGGGCGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCa                         <  2:508605/139‑1 (MQ=255)                                                                                    tATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCa                         >  1:226556/1‑139 (MQ=255)                                                                                         aacaaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATg                               <  1:1328406/128‑1 (MQ=255)                                                                                         aacaaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATg                               >  2:1328406/1‑128 (MQ=255)                                                                                           caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAGCGGCTGCAGGAGTTAAAAGACg                                                                                                <  1:547125/61‑1 (MQ=255)                                                                                           caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAGCGGCTGCAGGAGTTAAAAGACg                                                                                                >  2:547125/1‑61 (MQ=255)                                                                                           caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTc                  <  1:1082199/139‑1 (MQ=255)                                                                                              gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTg               >  2:1296670/1‑139 (MQ=255)                                                                                                gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTg                                       >  1:477322/1‑113 (MQ=255)                                                                                                gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTg                                       <  2:477322/113‑1 (MQ=255)                                                                                                gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGcc             <  1:195521/139‑1 (MQ=255)                                                                                                gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGcc             <  1:563739/139‑1 (MQ=255)                                                                                                   tAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg          <  2:1006318/139‑1 (MQ=255)                                                                                                   tAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg          <  2:226556/139‑1 (MQ=255)                                                                                                      aGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtgg       >  1:58870/1‑139 (MQ=255)                                                                                                        ttATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtggtg     <  2:58870/139‑1 (MQ=255)                                                                                                        ttATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtggtg     <  1:1296670/139‑1 (MQ=255)                                                                                                          aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCt                    >  2:351180/1‑122 (MQ=255)                                                                                                          aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCt                    <  1:351180/122‑1 (MQ=255)                                                                                                           tCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACCAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCaa  >  2:952881/1‑139 (MQ=255)                                                                                                               cACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTgg                                                                                          <  2:1113058/47‑1 (MQ=255)                                                                                                               cACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTgg                                                                                          >  1:1113058/1‑47 (MQ=255)                                                                                                                aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg          <  1:809353/126‑1 (MQ=255)                                                                                                                aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg          >  2:809353/1‑126 (MQ=255)                                                                                                                    gCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtggtg     >  1:127743/1‑127 (MQ=255)                                                                                                                    gCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtggtg     <  2:127743/127‑1 (MQ=255)                                                                                                                         cGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                       <  1:35974/40‑1 (MQ=255)                                                                                                                         cGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                       >  2:35974/1‑40 (MQ=255)                                                                                                                         cGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg          >  2:724210/1‑117 (MQ=255)                                                                                                                         cGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAg          <  1:724210/117‑1 (MQ=255)                                                                                                                              ggAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtg        >  2:635894/1‑114 (MQ=255)                                                                                                                              ggAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtg        <  1:635894/114‑1 (MQ=255)                                                                                                                                     |                                                                                                                 ACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAA  >  NC_000913/1627493‑1627737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑