Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,744,740 C→T G428G (GGC→GGT mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,7400CT100.0% 102.5 / NA 33G428G (GGC→GGTmdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/14);  total (19/14)

TTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTT  >  NC_000913/1744625‑1744870
                                                                                                                   |                                                                                                                                  
ttACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCa                                                                                                                    <  2:746430/132‑1 (MQ=255)
ttACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCa                                                                                                                    >  1:746430/1‑132 (MQ=255)
              tACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCa                                                                                                                        >  1:1261005/1‑114 (MQ=255)
              tACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCa                                                                                                                        <  2:1261005/114‑1 (MQ=255)
                   ggTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc                                                                                          >  1:1016185/1‑139 (MQ=255)
                                     cGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCa                                                                        >  1:865549/1‑139 (MQ=255)
                                                  gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGTCGTCGGCAg                                                                                                                       <  2:762139/79‑1 (MQ=255)
                                                  gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGTCGTCGGCAg                                                                                                                       >  1:762139/1‑79 (MQ=255)
                                                     cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAc                                                        >  1:1241244/1‑139 (MQ=255)
                                                        aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt                                                                                                 >  1:1062063/1‑95 (MQ=255)
                                                        aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTAt                                                                                                 <  2:1062063/95‑1 (MQ=255)
                                                            aTCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                    >  1:560377/1‑120 (MQ=255)
                                                            aTCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                    <  2:560377/120‑1 (MQ=255)
                                                               tGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                              <  2:167094/139‑1 (MQ=255)
                                                               tGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                              <  2:1016185/139‑1 (MQ=255)
                                                                ggTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATGGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGc                                             >  1:205003/1‑139 (MQ=255)
                                                                  tCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCta                                           >  1:809413/1‑139 (MQ=255)
                                                                       gAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCa                                                                                                                        <  2:831928/57‑1 (MQ=255)
                                                                       gAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCa                                                                                                                        >  1:831928/1‑57 (MQ=255)
                                                                            tATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCgg                                                                                             >  1:803003/1‑79 (MQ=255)
                                                                            tATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCgg                                                                                             <  2:803003/79‑1 (MQ=255)
                                                                               ggggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCa                                                                                                                    >  1:625949/1‑53 (MQ=255)
                                                                               ggggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCa                                                                                                                    <  2:625949/53‑1 (MQ=255)
                                                                               ggggCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGgc                              <  2:865549/139‑1 (MQ=255)
                                                                                  gCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCa                           >  1:858216/1‑139 (MQ=255)
                                                                                  gCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCa                           >  1:662876/1‑139 (MQ=255)
                                                                                                 gATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                    <  2:510584/83‑1 (MQ=255)
                                                                                                 gATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                    >  1:510584/1‑83 (MQ=255)
                                                                                                      gCTTTATTATTGGTCTGACGTCGGCAGCCATTatgatgat                                                                                                          <  2:1241951/40‑1 (MQ=255)
                                                                                                      gCTTTATTATTGGTCTGACGTCGGCAGCCATTatgatgat                                                                                                          >  1:1241951/1‑40 (MQ=255)
                                                                                                      gCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                    >  2:1282075/1‑78 (MQ=255)
                                                                                                      gCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                    <  1:1282075/78‑1 (MQ=255)
                                                                                                           attattGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAttt  >  1:287480/1‑139 (MQ=255)
                                                                                                                   |                                                                                                                                  
TTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTT  >  NC_000913/1744625‑1744870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: