Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	169,645	A→G	Y721C (TAC→TGC)	fhuA →	ferrichrome outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	169,645	0	A	G	100.0%	128.6 / NA	36	Y721C (TAC→TGC)	fhuA	ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

TCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATG  >  NC_000913/169517‑169780                                                                                                                                 |                                                                                                                                        tCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                               >  1:172462/1‑139 (MQ=255) tCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                               <  2:159164/139‑1 (MQ=255)        aaCTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTa                                                                                                                        <  2:172462/139‑1 (MQ=255)             cTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACt                                                                                                                   <  2:673160/139‑1 (MQ=255)               ttAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTa                                                                                                                 >  1:362213/1‑139 (MQ=255)                   aGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             <  2:1170007/139‑1 (MQ=255)                                    tCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGc                                                                                                                                   >  1:1301997/1‑100 (MQ=255)                                    tCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGc                                                                                                                                   <  2:1301997/100‑1 (MQ=255)                                              gTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                               <  2:875539/94‑1 (MQ=255)                                              gTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAgc                                                                                                                               >  1:875539/1‑94 (MQ=255)                                              gTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTa                                                                                                                        <  1:1326770/101‑1 (MQ=255)                                              gTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTa                                                                                                                        >  2:1326770/1‑101 (MQ=255)                                                        tATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       >  2:323720/1‑92 (MQ=255)                                                        tATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       <  1:323720/92‑1 (MQ=255)                                                             tCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccgcaacc                                                                   >  1:76152/1‑139 (MQ=255)                                                               tGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCtt                                                                 >  2:1116606/1‑139 (MQ=255)                                                                      aGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       <  1:342948/78‑1 (MQ=255)                                                                      aGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTaa                                                                                                                       >  2:342948/1‑78 (MQ=255)                                                                      aGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTc                                                          >  2:688523/1‑139 (MQ=255)                                                                           gCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccgcaac                                                                    >  1:418812/1‑124 (MQ=255)                                                                           gCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccgcaac                                                                    <  2:418812/124‑1 (MQ=255)                                                                                              ggCGCTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGAt                                  >  2:832201/1‑139 (MQ=255)                                                                                                  cTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCttt                                                                                                                         <  1:85417/48‑1 (MQ=255)                                                                                                  cTGCATGTTAACAACCTGTTCGATCGTGAATGCGTCGCCAGCTGCttt                                                                                                                         >  2:85417/1‑48 (MQ=255)                                                                                                               acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGa                                   <  1:322834/121‑1 (MQ=255)                                                                                                               acCTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGa                                   >  2:322834/1‑121 (MQ=255)                                                                                                                ccTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGcc                         >  2:1258375/1‑130 (MQ=255)                                                                                                                ccTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGcc                         <  1:1258375/130‑1 (MQ=255)                                                                                                                 cTGTTCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcacca               >  2:690546/1‑137 (MQ=255)                                                                                                                    ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             <  2:58077/42‑1 (MQ=255)                                                                                                                    ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGgc                                                                                                             >  1:58077/1‑42 (MQ=255)                                                                                                                    ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTg                                                                              >  2:197839/1‑73 (MQ=255)                                                                                                                    ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTg                                                                              <  1:197839/73‑1 (MQ=255)                                                                                                                    ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt            <  1:832201/139‑1 (MQ=255)                                                                                                                    ttCGATCGTGAATGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCACAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt            >  2:142501/1‑139 (MQ=255)                                                                                                                              aaTGCGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATg  <  1:1116606/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        TCCGGCTAACTCCTTTAAAGTGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATG  >  NC_000913/169517‑169780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑