Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,819,569 A→G G79G (GGT→GGC)  chbA ← N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,5690AG100.0% 198.5 / NA 56G79G (GGT→GGC) chbAN,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/25);  total (31/25)

GCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACG  >  NC_000913/1819438‑1819702
                                                                                                                                   |                                                                                                                                     
gCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGc                                                                                                                                >  1:465109/1‑139 (MQ=255)
      tcatcGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          <  2:465109/139‑1 (MQ=255)
              tCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCa                                                                                                                  >  2:1010475/1‑139 (MQ=255)
                cTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                >  2:119106/1‑139 (MQ=255)
                cTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                >  2:1115403/1‑139 (MQ=255)
                cTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                >  1:780691/1‑139 (MQ=255)
                   aTGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGcc                                                                                                                      >  2:617643/1‑130 (MQ=255)
                   aTGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGcc                                                                                                                      <  1:617643/130‑1 (MQ=255)
                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCa                                                                                                                           <  1:429263/118‑1 (MQ=255)
                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCa                                                                                                                           >  2:429263/1‑118 (MQ=255)
                                            aattaattCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCttca                                                                                    >  2:125649/1‑139 (MQ=255)
                                                       tAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             >  1:585728/1‑135 (MQ=255)
                                                       tAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             <  2:585728/135‑1 (MQ=255)
                                                       tAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCAt                                                                         >  2:129805/1‑139 (MQ=255)
                                                           cAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGTGCTTCATTCAATGCCATCCGt                                                                     <  1:145373/139‑1 (MQ=255)
                                                              ttCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGAc                                                                  >  2:612976/1‑139 (MQ=255)
                                                                       aaGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatc                                                         <  1:612976/139‑1 (MQ=255)
                                                                        aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGt                                                                                                       >  1:558883/1‑92 (MQ=255)
                                                                        aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGt                                                                                                       <  2:558883/92‑1 (MQ=255)
                                                                        aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatca                                                        >  1:1207746/1‑139 (MQ=255)
                                                                             ggACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCt                                                                                       <  1:134096/103‑1 (MQ=255)
                                                                             ggACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCt                                                                                       >  2:134096/1‑103 (MQ=255)
                                                                                 gTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtg                                               <  2:780691/139‑1 (MQ=255)
                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGt                                                                                                       >  1:651128/1‑81 (MQ=255)
                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatc                                                         <  2:238715/127‑1 (MQ=255)
                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatc                                                         >  1:238715/1‑127 (MQ=255)
                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgct                                             >  1:784532/1‑139 (MQ=255)
                                                                                   cATTAAATGATCCTGAGCGTGCACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGt                                                                                                       <  2:651128/81‑1 (MQ=255)
                                                                                     ttAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGcc                                                                                                                               <  1:478654/55‑1 (MQ=255)
                                                                                     ttAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGcc                                                                                                                               >  2:478654/1‑55 (MQ=255)
                                                                                            aTCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCaaaa                                    <  1:1115403/139‑1 (MQ=255)
                                                                                              ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                >  1:282922/1‑61 (MQ=255)
                                                                                              ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                <  2:282922/61‑1 (MQ=255)
                                                                                              ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATc                                  >  2:419212/1‑139 (MQ=255)
                                                                                               cTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCg                                 >  1:1312083/1‑139 (MQ=255)
                                                                                               cTGAGCGTGGACCAGCACCAAACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGttt                                                                                                          <  2:1090808/66‑1 (MQ=255)
                                                                                               cTGAGCGTGGACCAGCACCAAACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGttt                                                                                                          >  1:1090808/1‑66 (MQ=255)
                                                                                                tGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGc                                <  1:129805/139‑1 (MQ=255)
                                                                                                    cGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCtt                                                                                      <  2:134009/81‑1 (MQ=255)
                                                                                                    cGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCtt                                                                                      >  1:134009/1‑81 (MQ=255)
                                                                                                      tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCt                          >  1:576259/1‑139 (MQ=255)
                                                                                                       ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          >  2:560973/1‑42 (MQ=255)
                                                                                                       ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          <  1:560973/42‑1 (MQ=255)
                                                                                                       ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCtt                         >  2:1304532/1‑139 (MQ=255)
                                                                                                        gACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTc                        >  2:1061767/1‑139 (MQ=255)
                                                                                                            aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatcat                                                       <  2:388227/104‑1 (MQ=255)
                                                                                                            aGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCcatcat                                                       >  1:388227/1‑104 (MQ=255)
                                                                                                                     cTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTg           <  2:576259/139‑1 (MQ=255)
                                                                                                                        aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                <  2:25969/35‑1 (MQ=255)
                                                                                                                        aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAAt                                                                                                                >  1:25969/1‑35 (MQ=255)
                                                                                                                         cTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGc       <  1:119106/139‑1 (MQ=255)
                                                                                                                           ttCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGttt                                                                                                          <  2:1164572/38‑1 (MQ=255)
                                                                                                                           ttCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGttt                                                                                                          >  1:1164572/1‑38 (MQ=38)
                                                                                                                              aTCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGt                                                                                                       >  2:133572/1‑38 (MQ=255)
                                                                                                                              aTCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGt                                                                                                       <  1:133572/38‑1 (MQ=255)
                                                                                                                              aTCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACg  <  2:784532/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
GCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACG  >  NC_000913/1819438‑1819702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑